Durian Seed Flour (Durio zibethinus Murr) as an Alternative Medium for Fungal Growth

Tika Afriani, Azimatur Rahmi, Putri Agiel Ramadhani, Rahmadaniah Rahmadaniah

Abstract


This study aimed to evaluate the potential of durian seed flour (Durio zibethinus Murr) as an alternative medium for the growth of Candida albicans and Aspergillus niger. The bleaching process utilized sodium metabisulfite at varying concentrations (600 ppm, 800 ppm, 1000 ppm) and immersion durations (15, 20, 25 minutes). The bleached samples were dried at 70℃ for 3 hours. Durian seed flour media were formulated into three variants and compared with Potato Dextrose Agar (PDA) as the control medium. The activity test for Candida albicans used the pour plate method with incubation for 48 hours at 37℃, while Aspergillus niger was tested using the agar block method and incubated for 72 hours under the same conditions. Immersion duration significantly affected the flour's color, with longer durations producing whiter flour. Higher drying temperatures enhanced water evaporation efficiency, resulting in an average moisture content of 10.56%, compliant with national standards. The best medium formulation was F1 (10 g durian seed flour, 2 g sugar, 1.5 g agar, 0.25 g micronutrients, 100 mL distilled water), yielding 3.6 × 10ⷠCFU/mL of Candida albicans colonies and an Aspergillus niger growth diameter of 28.5 mm after 72 hours of incubation. However, sporulation of Aspergillus niger was better on PDA media. Durian seed flour shows potential as an alternative fungal growth medium.

Keywords


Aspergillus niger; Candida albicans; Durian seed flour; Fungal growth medium.

Full Text:

PDF

References


Abdillah, H., Achmad, A., & Setiawan, A. (2015). Pengaruh kadar glukosa dan waktu inokulasi pada optimasi pembuatan enzim selulose dengan menggunakan jamur Aspergillus niger dan substrat kertas. 1988, 186–192.

Afriani, T., Rahmi, A., & Pengesti, R. (2022). Utilization of durian seed flour (Durio zibethinus Murr) as a medium of mushroom growth. World Journal of Pharmaceutical Research, 11(16), 1667–1675.

Damayanti, A., Putri, R. D. M., Megawati, M., Siami, D. H., & Fitriani, Z. (2020). Peningkatan nilai tambah biji durian (Durio zibethinus) dan biji rambutan (Nephelium lappaceum) menjadi keripik. ABDIMAS: Jurnal Pengabdian Masyarakat, 3(2), 264–273.

Etiek. (2020). Pertumbuhan koloni Aspergillus niger pada media agar tepung beras dektrosa dengan metode dilusi. 1–4.

Fadhilah, F. R. (n.d.). Identification of Aspergillus sp growth on white bread against storage temperature. 10(2), 92–103.

Faradiana. (2016). Pemanfaatan sumber karbohidrat yang berbeda (umbi suweg dan umbi kimpul) sebagai substitusi media PDA (Potato Dextrose Agar) untuk pertumbuhan jamur. 53(9), 1689–1699.

Haryani, K., & Santosa, T. B. (2016). Pemutihan tepung porang (Amorphophallus onchophyllus) menggunakan natrium metabisulfit dan vitamin C. In Seminar Nasional Terapan Riset Inovatif Semarang (Vol. 1).

Hasanah, U. (2017). Mengenal aspergillosis, infeksi jamur genus Aspergillus. Jurnal Keluarga Sehat Sejahtera, 15(2), 76–86.

Hevira, et al. (2021). Pembuatan biofoam berbahan dasar ampas tebu dan whey. Jurnal Kimia dan Kemasan, 43(2), 75–81.

Kiptiah, M., Amalia, R. R., & Hayati, M. (2019). Substitusi tepung pisang kepok dalam pembuatan produk soes kering pisang kepok. Jurnal Teknologi Agro-Industri, 6(2), 108.

Lastari, A. N., Anandito, R. B. K., & Siswanti, S. (2016). Pengaruh konsentrasi natrium metabisulfit (Naâ‚‚Sâ‚‚Oâ‚…) dan lama perendaman terhadap karakteristik tepung kecambah kedelai. Jurnal Teknosains Pangan, 5(2).

Lopulalan, C. G. (2016). Pengaruh penambahan tepung ketan terhadap sifat kimia dodol tepung biji durian. Jurnal Agroforestri, 9(3), 195–204.

Magfirah, A., & Fitriana, S. M. (2020). Uji aktivitas antioksidan isolat fungi endofit daun galing-galing (Cayratia trifolia L.) dengan metode 1,1-Diphenyl-2-Picrylhdrazil (DPPH). Akrab Juara, 5(1), 43–54.

Mujipradhana, V. N., Wewengkang, D. S., & Suryanto, E. (2018). Aktivitas antimikroba dari ekstrak Ascidian herdmania momus pada mikroba patogen manusia. Pharmacon, 7(3), 338–347.

Nugroho, B. A., & Sari, D. M. (2019). Peningkatan kualitas tepung aren pada sentra industri kecil soun Klaten melalui variasi kondisi proses pemutihan. In B. S. Ariwibawa (Ed.), (Vol. 4).

Ornay, A. K. D., Prehananto, H., & Dewi, A. S. S. (2017). Daya hambat pertumbuhan Candida albicans dan daya bunuh Candida albicans ekstrak daun kemangi (Ocimum sanctum L.). Jurnal Wiyata, 4(1), 78–83.

Qurrohman, M. S. N. A. F., & M. T. (2021). Efektivitas variasi konsentrasi tepung talas (Colocasia esculenta (L.) Schott) sebagai media alternatif pertumbuhan jamur Candida albicans. 116(1), 32–47.

Simanjuntak, S., Nugroho, W. A., & Yulianingsih, R. (2014). Pengaruh suhu pengeringan dan konsentrasi natrium metabisulfit (Na₂S₂O₅) terhadap sifat fisik-kimia tepung biji durian (Durio zibethinus). Jurnal Bioproses Komoditas Tropis, 2(2), 91–99.

Siregar, K. (2016). Pendugaan umur simpan tepung biji durian (Durio zibethinus) dengan menggunakan persamaan Arrhenius. Program Studi Teknik Pertanian, Fakultas Pertanian, Universitas Syiah Kuala, 9(April), 74–88.

Sundari, W., Wisrakarmila, Marlina, D., & Faizah. (2021). Pemanfaatan biji mangga arum manis (Mangifera indica L.) sebagai media alternatif pertumbuhan Candida albicans dan Aspergillus sp. Jurnal Pengelolaan Laboratorium Pendidikan, 3(1), 14–17.




DOI: https://doi.org/10.14421/biomedich.2025.141.435-441

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2025 Tika Afriani, Azimatur Rahmi, Putri Agiel Ramadhani, Rahmadaniah



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC