Evaluation of the Efficacy of Miira-Cell, a Novel Nutraceutical, in Reducing High-Fat Diet-Induced Hypercholesterolemia in Wistar Rats

Chioma Cordelia Ugwu, Stanley Obinna Ezeadichie, Okey John Nnamani, Osah Martins Onwuka, Chioma Marylyn Adilieje

Abstract


Hypercholesterolemia is a major risk factor for cardiovascular diseases, often exacerbated by high-fat diets. Miira-Cell, a novel nutraceutical, has been developed to address this issue. This study evaluates the efficacy of Miira-Cell in mitigating hypercholesterolemia induced by high-fat diet in Wistar rats. Acute toxicity testing showed that Miira-Cell was safe, with an LD50 greater than 5000 mg/kg. Thirty Wistar rats were divided into six groups: normal control, high-fat diet control, and three groups receiving high-fat diets supplemented with low, medium, and high doses of Miira-Cell, respectively. A sixth group received Rosuvastatin as a positive control. After a 14-day treatment period following a 21-day high-fat diet, lipid profiles, liver biomarkers, oxidative stress markers, and cardiac tissue histopathology were assessed. The results showed that Miira-Cell significantly reduced triglycerides, VLDL, and total cholesterol in a dose-dependent manner, similar to Rosuvastatin. Both Miira-Cell and Rosuvastatin also lowered LDL levels. However, all treatment groups showed decreased HDL levels, which may indicate potential effects on HDL metabolism. Miira-Cell demonstrated hepatoprotective properties by reducing liver enzyme levels and oxidative stress. Histopathological analysis revealed tissue damage in the negative control group, while tissue integrity was preserved in Miira-Cell and Rosuvastatin-treated groups. In conclusion, Miira-Cell shows potential as a therapeutic agent for hypercholesterolemia and associated liver diseases, although further research is necessary to elucidate its mechanisms and clinical applicability.

Keywords


Hypercholesterolemia; Miira-Cell; Nutraceutical; High-fat diet; Lipid profile; Oxidative stress; Hepatoprotection; Rosuvastatin

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References


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DOI: https://doi.org/10.14421/biomedich.2026.151.53-59

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Copyright (c) 2026 Chioma Cordelia Ugwu, Stanley Obinna Ezeadichie, Okey John Nnamani, Osah Martins Onwuka, Chioma Marylyn Adilieje



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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