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Potential Effect of Orthosiphon aristatus Leaf Extract in Improving Collagen 1 Alpha 1 Expression in Hyperglycemia-Induced Rats
Abstract
Diabetes mellitus involves many mechanisms, the collagen metabolism pathway with very complex interactions and its regulation can contribute to the development of diabetes complications. The collagen 1 alpha 1 (COL1A1) gene plays an important role in the diabetes melitus pathway in adipose tissue by regulating adipocyte function, insulin resistance, and glucose metabolism. COL1A1 expression is positively correlated with insulin resistance and negatively correlated with insulin sensitivity and is identified as a potential therapeutic target for type 2 diabetes. The cat's whisker plant (Ortosiphon aristatus) pharmacologically has hypoglycemic activity and increases insulin expression. This study aims to observe the potential of cat whisker leaf extract (Ortosiphon aristatus) in increasing the expression of the COL1A1 gene and is protective against increased blood sugar levels in hyperglycemia-induced Rattus norvegicus. The experimental design with pretest and posttest controlled group design was carried out by dividing 18 test animals into six groups of non-diabetic negative control, diabetic negative control, metformin as positive control, doses of 100, 200, and 400 mg/kgBW. Induction of hyperglycemia with streptozotocin 65 mg/kgBW and nicotinamide 230 mg/kgBW peritoneal injection. The extract treatment is given for 14 days. Blood sugar level measurements were carried out at the treatment’s beginning and end. Adipose tissue was taken to examine COL1A1 gene expression by real time PCR technique by calculating the ratio of the cycle of tresh-hold COL1A1 to the internal ?-actin gene. The results of Kruskall Wallis test showed no significant difference in COL1A1 gene expression between groups and the Spearmen correlation test showed no relationship between blood sugar levels and COL1A1 gene expression (p>0.05). There was a decrease in blood sugar levels before and after treatment for all treatment groups.
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Arifah, F. H., Nugroho, A. E., Rohman, A., & Sujarwo, W. (2022). A review of medicinal plants for the treatment of diabetes mellitus: The case of Indonesia. South African Journal of Botany, 149, 537–558. https://doi.org/10.1016/j.sajb.2022.06.042.
Cruz, B., Flores, R. J., Uribe, K. P., Espinoza, E. J., Spencer, C. T., Serafine, K. M., Nazarian, A., & O’Dell, L. E. (2019). Insulin modulates the strong reinforcing effects of nicotine and changes in insulin biomarkers in a rodent model of diabetes. Neuropsychopharmacology, 44(6), 1141–1151. https://doi.org/10.1038/s41386-018-0306-3.
Devos, H., Zoidakis, J., Roubelakis, M. G., Latosinska, A., & Vlahou, A. (2023). Reviewing the Regulators of COL1A1. International Journal of Molecular Sciences, 24(12), 10004. https://doi.org/10.3390/ijms241210004.
Harijanto, E. A., & Dewajanti, A. M. (2017). Optimalisasi Pemberian Streptozotocin Beberapa Dosis terhadap Peningkatan Kadar Gula Darah Tikus Sprague dawley. 23(63).
Khan, M. A. B., Hashim, M. J., King, J. K., Govender, R. D., Mustafa, H., & Al Kaabi, J. (2019). Epidemiology of Type 2 Diabetes – Global Burden of Disease and Forecasted Trends: Journal of Epidemiology and Global Health, 10(1), 107. https://doi.org/10.2991/jegh.k.191028.001
Lin, G., Wan, X., Liu, D., Wen, Y., Yang, C., & Zhao, C. (2021). COL1A1 as a potential new biomarker and therapeutic target for type 2 diabetes. Pharmacological Research, 165, 105436. https://doi.org/10.1016/j.phrs.2021.105436
Maulana, F., Muhammad, A. A., Umar, A., Mahendra, F. R., Musthofa, M., & Nurcholis, W. (2022). Profiling Metabolites through Chemometric Analysis in Orthosiphon aristatus Extracts as ?-Glucosidase Inhibitory Activity and In Silico Molecular Docking. Indonesian Journal of Chemistry, 22(1), 501. https://doi.org/10.22146/ijc.71334
Munjiati, N. E. (2021). Pengaruh Pemberian Streptozotocin Dosis Tunggal terhadap Kadar Glukosa Tikus Wistar (Rattus norvegicus). Meditory : The Journal of Medical Laboratory, 9(1), 62–67. https://doi.org/10.33992/m.v9i1.1330
Ojo, O. A., Ibrahim, H. S., Rotimi, D. E., Ogunlakin, A. D., & Ojo, A. B. (2023). Diabetes mellitus: From molecular mechanism to pathophysiology and pharmacology. Medicine in Novel Technology and Devices, 19, 100247. https://doi.org/10.1016/j.medntd.2023.100247
Septiana, E., Rizka, N. M., Yadi, Y., & Simanjuntak, P. (2021). Antidiabetic Activity of Extract Combination of Orthosiphon aristatus and Oryza sativa L. var glutinosa. Borneo Journal of Pharmacy, 4(3), 202–209. https://doi.org/10.33084/bjop.v4i3.2154
Thinn, N. W., Oo, Z. K., & Maw, S. S. (2018). Investigation of Hypoglycaemic Activity of Some Myanmar Medicinal Plants. International Journal of Technical Research & Science, 3(03), 104–108. https://doi.org/10.30780/IJTRS.V3.I3.2018.011
Wahidin, M., Achadi, A., Besral, B., Kosen, S., Nadjib, M., Nurwahyuni, A., Ronoatmodjo, S., Rahajeng, E., Pane, M., & Kusuma, D. (2024). Projection of diabetes morbidity and mortality till 2045 in Indonesia based on risk factors and NCD prevention and control programs. Scientific Reports, 14(1), 5424. https://doi.org/10.1038/s41598-024-54563-2
Waras Nurcholis, Fachrur Rizal Mahendra, Milanda Fiorella Gultom, Safira Khoirunnisa, Mayang Anggita Cahya Kurnia, & Hamdan Hafizh Harahap. (2022). Phytochemical, Antioxidant and Antibacterial Screening of Orthosiphon stamineus Leaf Extract Two Phenotypes. Jurnal Jamu Indonesia, 7(3), 121–129. https://doi.org/10.29244/jji.v7i3.280
Weng, X., Lin, D., Huang, J. T. J., Stimson, R. H., Wasserman, D. H., & Kang, L. (2020). Collagen 24 ?1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue. International Journal of Molecular Sciences, 21(16), 5738. https://doi.org/10.3390/ijms21165738
DOI: https://doi.org/10.14421/biomedich.2025.141.357-361
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