Email this article 
The New Technology for In Vitro Culture with Induction of Nanobubbles (NBsN2 and NBsO2) in Cattleya sp.
Abstract
The growth and development of orchids take a relatively long time, while the demand for high-quality orchids continues to increase significantly. Tissue culture is a technique of isolating plant parts in organs, tissues, and cells and then culturing these plants on artificial media in a sterile environment. Tissue culture techniques can reproduce plants in a relatively short time, with the same properties and quality, so efforts to optimize tissue culture with technology are the right solution, one of which is using nanogenerator technology that produces Nanobubbles (NBs). NBs are one of the nanotechnologies that are ?100 nm in size with induced gas needed by plants. This study aims to determine the effectiveness of growth and development from the induction of NBsN2 and NBsO2 in Cattleya sp. planlet in vitro culture. This research method uses in vitro culture with a Randomized Group Design. The results of the MsO treatment showed higher weight than other treatments, with a total plant weight value of 0.3550 g and a total plant height value of 1.3983 cm. Murashige-Skoog + NBsNitrogen (MsN) treatment showed higher root length than other treatments with a total value of 1.2367 cm. In the treatment of Ms, MsO, OJ and NJ, a live percentage of 83-100% was observed. The statistical test results indicate that NBs positively affect Cattleya sp. plantlets. This effect includes increasing plant weight, height, and root length and promoting new shoots growth. Additionally, nitrogen supplements play a role in colour changes, indicating the plant's health and photosynthetic efficiency. In the treatment with Induction O2, the initial colour changes from Strong Yellow Green (141 D) to Strong Yellowish Green (141 C), while in the treatment with Induction NBsN2, the initial colour changes from Strong Yellow Green (141 D) to Deep Yellow Green (141 B).
Keywords
Full Text:
PDFReferences
Ahmed, A.K.A., Shi, X., Hua, L., Manzueta, L., Qing, W., Marhaba, T., & Zhang, W. (2018). Influences of Air, Oxygen, Nitrogen, and Carbon Dioxide Nanobubbles on Seed Germination and Plant Growth. Journal of Agricultural and Food Chemistry, 66 (20), 5117-5124. Doi: https://doi.org/10.1021/acs.jafc.8b00333
Han, Z., Chen, H., He, C., Dodbiba, G., Otsuki, A., Wei, Y., & Fujita, T. (2023). Nanobubble size distribution measurement by interactive force apparatus under an electric field. Scientific Reports, 13(1), 1-12. Doi: https://doi.org/10.3390/nano12020237
Krisna, B., Putra, E.T.S., Rogomulyo, R., & Kastono, D. (2017). Effect of oxygen enrichment on root growth and yield of curly lettuce (Lactuca sativa L.) in floating raft hydroponics. Journal of Vegetalika, 6(4),14-17. Doi: https://doi.org/10.22146/VEG.30900
Kusuma, R., Samsurianto, S., Azizah, W., & Yuliatin, E. (2023). Growth Response of Protocorm Like Bodies (PLB) of Sugarcane Orchid to Modification of Murashige and Skoog (MS) Media in vitro. Paspalum: Scientific Journal of Agriculture, 11(1), 42-50. Doi: https://doi.org/10.35138/paspalum.v11i1.538
Latifah, R., Suhermiatin, T., & Ermawati, N. (2017). Optimization of Cattleya Plantlet Growth Through a Combination of Murashige-Skoog Media Strength and Organic Materials. Agriprima: Journal of Applied Agricultural Sciences, 1(1), 59-62. Doi: https://doi.org/10.25047/agriprima.v1i1.20
Nisa, N.A., Rahayu, T., Jayanti, G.E. (2021). The Utility of BAP and Coconut Water in VW Medium on the Organogenesis of Dendrobium sp. Metamorfosa: Journal of Biological Sciences, 8(2), 298-303. Doi: https://doi.org/10.24843/metamorfosa.2021.v08.i02.p14
Patti, P.S., Kaya, E., & Silahooy, C. (2013). Analysis of soil nitrogen status in relation to N uptake by paddy rice plants in Waimital village, Kairatu District, West Seram Regency. Journal of Agrologia, 2(1), 51-58. Doi: https://dx.doi.org/10.30598/a.v2i1.278
Rahayu, T., Jayanti, G. E., & Hayati, A. (2023). Induction of Nanobubbles (NBs) for growth of Dendrobium Imelda Marina Masagung x Bumi Menangis orchids. Journal of Metamorphosa, 10(1), 126-132. Doi: https://doi.org/10.24843/metamorfosa.2023.v10.i01.p13
Wang, Y., Wang, S., Sun, J., Dai, H., Zhang, B., Xiang, W., Hu, Z., Li, P., Yang, J., & Zhang, W. (2021). Nanobubbles promote nutrient utilization and plant growth in rice by upregulating nutrient uptake genes and stimulating growth hormone production. Science of the Total Environment, 800(1), 1-9. Doi: https://doi.org/10.1016/j.scitotenv.2021.149627
Wulandari, D. C., Rahayu, Y. S., & Ratnasari, E. (2014). Effect of Gibberellin Hormone on Parthenocarpy Fruit Formation in Cucumber Plants of Mercy Variety. LenteraBio, 3(1), 27-32. Doi:
Yachya, A., Sopandi, T., Slamet, P., Binawati, D.K., Ngadiani., Sukarjati., Ajiningrum, P.S., Andriani, V. (2022). Plants in East Java SMS Biology Mgmp Teachers. Journal of Penamas Adi Buana, 6(1), 31-36. Doi: https://doi.org/10.36456/penamas.vol6.no01.a5498
Yasmin, Z. F. (2018). Nursery (tissue culture to enlargement) of Phalaenopsis Orchid at Hasanudin Orchids, East Java Nursery. Agrohorti Bulletin Journal, 6(3), 430-439. Doi: https://doi.org/10.29244/agrob.v6i3.21113
Firmansyah, M. I., Rahayu, & Jayati, G. E., Agisimanto, D. (2023). Comparative Study of Naphtalene Acetic Acid (NAA) Concentration Variations on the Growth of Dendrobium sp. Orchid on White and Black Moss Media. Journal of Metamorphosa, 10(1), 292-301. Doi: https://doi.org/10.24843/metamorfosa.2023.v10.i02.p13
Ai, N.S., & Torey, P. (2013). Root morphological characters as water-deficit indicators in plants. Journal of Bioslogos, 3(1), 32-39. Doi: https://doi.org/10.35799/jbl.3.1.2013.3466
Rahma, S., Rahayu, T., & Hayati, A. (2018). Study of Organic Material Addition in VW Planting Media on Organogenesis of Dendrobium Orchid in Vitro. E-Journal of Known Nature 1(1), 93 - 103. Doi: https://doi.org/10.33474/j.sa.v1i1.1392
DOI: https://doi.org/10.14421/biomedich.2024.132.459-465
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Moch Mustakim, Azis Mawardi, Lina Purnama Dewi, Nela Vede Rhofa Putri, Tintrim Rahayu, Gatra Ervi Jayanti
Biology, Medicine, & Natural Product Chemistry |
|
