Histopathological Profile of Wistar Rat Kidney in High-Fat Diet Model Treated with a Combination of Tea and Mango Mistletoe Extracts

Rianda Siwi Cholida, Nour Athiroh Abdoes Sjakoer, Ari Hayati

Abstract


A high-fat diet (HFD) can cause kidney damage through oxidative stress and inflammation. This study aimed to analyze the histopathology of the kidneys in rats given HFD after administering a combination of tea mistletoe leaf extract (Scurrula atropurpurea) and mango mistletoe (Dendrophthoe pentandra) (MESA-DP). Using a randomized pretest-posttest control group design, the rats were divided into a negative control group (G1), an HFD control group (G2), and three treatment groups with combined doses of MESA-DP: 50, 100, and 200 mg/kg body weight (G3, G4, G5). During the 37-day treatment period, microscopic examinations were conducted. The results showed that (G2) experienced an increase in fat accumulation (12%) and glomerulus diameter (1783.1 micrometer), compared to G1 (6%; 1507.5 micrometer). G3 actually worsened the condition (22%; 2003.1 micrometer). G4 successfully reduced fat accumulation to 9% and glomerulus diameter to 1689.4 micrometer, while G5 approached normal levels (7%; 1674.4 micrometer). ANOVA tests on the kidney fat area and glomerulus diameter showed p=0.018 (p<0.05), indicating significant differences among the groups. These results demonstrate that the combination of MESA-DP at a dose of 100 mg/kg body weight (G4) can balance the side effects of HFD on kidney tissue and minimize inflammatory side effects in the glomerulus

Keywords


High-fat diet; histopathology; kidney; mango mistletoe; tea mistletoe

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DOI: https://doi.org/10.14421/biomedich.2026.151.1621-1628

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Copyright (c) 2026 Rianda Siwi Cholida, Nour Athiroh Abdoes Sjakoer, Ari Hayati



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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