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Physicochemical Kinetic Evaluation and Biological Activities of Wild Horsetail Extract
Abstract
The objective of this study is to evaluate the physicochemical, kinetic and bioactive characteristics of the alcoholic extract of Wild Horsetail. The total amount of phenolic and flavonoid was calculated and the antioxidant capacity was evaluated with the help of DPPH and ABTS tests. Potential for antidiabetic ability was also studied through the inhibition of alpha-amylase as well as alpha-glucosidase. In addition, the kinetics of trapping DPPH free radicals were studied. The chemical analysis revealed the presence of a variety of bioactive compounds, the major one being scoparone (26.45%) followed by phytol (7.43%) and methyleugenol (6.52%). Also, the total phenolic and flavonoid contents exhibited high amounts, specifically, 186.43 ± 4.18 mg gallic acid equivalent/g extract and 97.87 ± 2.66 mg quercetin equivalent/g extract, respectively. The extract displayed antioxidant capacity with IC50 of 71.37 ± 2.16 and 58.42 ± 1.87 µg/ml in DPPH and ABTS test respectively. It also showed significant inhibition of alpha-amylase and alpha-glucosidase enzymes with IC50 values of 92.78 ± 2.34 and 68.54 ± 1.92 µg/ml respectively. Based on the kinetic study, it was found that the DPPH free radical scavenging activity was described by a pseudo-first-order kinetic model. As a confirmation of the wild horsetail's phenolic and flavonoid content, ethanolic extract has a high concentration. These compounds have antioxidant ability and modulating effect on enzymes related to carbohydrate metabolism which can be incorporated into natural formulations. Such preparations can be used in pharmaceuticals and as dietetic supplements to prevent oxidative stress and assist in the treatment of diabetes.
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Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (4th ed.). Allured Publishing Corporation.
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30. doi.org/10.1016/S0023-6438%2895%2980008-5
Chang, C., Yang, M., Wen, H., & Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178–182.
?orkovi?, I., Gašo-Soka?, D., Pichler, A., Šimunovi?, J., & Kopjar, M. (2022). Dietary polyphenols as natural inhibitors of ?-amylase and ?-glucosidase. Life, 12(11), 1692.?
Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313–7352. https://doi.org/10.3390/molecules15107313
Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313–7352. doi.org/10.3390/molecules15107313
Kim, Y. M., Jeong, Y. K., Wang, M. H., Lee, W. Y., & Rhee, H. I. (2005). Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition, 21(6), 756–761. https://doi.org/10.1016/j.nut.2004.10.014
Kim, Y. M., Jeong, Y. K., Wang, M. H., Lee, W. Y., & Rhee, H. I. (2005). Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition, 21(6), 756–761.doi.org/10.1016/j.nut.2004.10.014
McCue, P., & Shetty, K. (2004). Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pacific Journal of Clinical Nutrition, 13(1), 101–106.
McCue, P., & Shetty, K. (2004). Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pacific Journal of Clinical Nutrition, 13(1), 101–106.
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290–4302. https://doi.org/10.1021/jf0502698
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. doi.org/10.1016/S0891-5849%2898%2900315-3
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178. doi.org/10.1016/S0076-6879%2899%2999017-1
Sok Yen, F., Shu Qin, C., Tan Shi Xuan, S., Jia Ying, P., Yi Le, H., Darmarajan, T., ... & Salvamani, S. (2021). Hypoglycemic effects of plant flavonoids: a review. Evidence?Based Complementary and Alternative Medicine, 2021(1), 2057333.?
Zhang, H., Chen, F., Wang, X., & Yao, H. (2017). Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(8), 833–839. doi.org/10.1016/j.foodres.2005.03.007
DOI: https://doi.org/10.14421/biomedich.2026.151.1459-1464
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Copyright (c) 2026 Baydaa Awad Kadhim, Fouad Razzaq Al-Burki, Assel Majeed Hammeed
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