Email this article 
Morphological and Cytological Characteristics of Tomato Plants (Solanum lycopersicum L.) Induced by Tapak Dara (Catharanthus roseus (L.) G. Don) Extract
Abstract
Polyploidy is commonly induced using antimitotic agents such as colchicine, which is imported and relatively expensive, therefore, alternative antimitotic agents derived from local resources are needed. There are three main classes of antimitotic agents: taxanes, vinca alkaloids, and colchicine. Taxanes are obtained only from the bark of the western yew plant, colchicine is derived from Colchicum autumnale L., and vinca alkaloids are obtained from the tapak dara plant (Catharanthus roseus). Therefore, the tapak dara plant has great potential as a source of antimitotic agents other than colchicine. This study aimed to determine the effect of tapak dara flower extract on the morphological and cytological characteristics of tomato plants (Solanum lycopersicum L.). The research used a completely randomized design with four treatment levels and five replications: P0 = control treatment, P1 = 0.05% concentration, P2 = 0.1% concentration, and P3 = 0.15% concentration, with a soaking duration of 10 hours. The observed parameters included germination percentage (%), plant height, number of leaves, number of flowers, and chromosome number. Data were analyzed using one-way ANOVA followed by Duncan’s multiple range test at the 5% significance level. The results showed significant effects on the number of leaves, number of flowers, and chromosome number. Treatment P1, with a concentration of 0.05%, was the best treatment.
Keywords
Full Text:
PDFReferences
Ainurrohmah, C., Biologi, I. J., Matematika, F., Pengetahuan, I., Universitas, A., & Surabaya, N. (2020). Perbandingan Efektivitas Ekstrak Etanolik Umbi Kembang Sungsang (Gloriosa superba) dan Daun Tapak Dara (Catharanthus roseus) sebagai Substitusi Kolkisin. 9(2), 158–167. https://journal.unesa.ac.id/index.php/lenterabio/index.
Banyal, A., Tiwari, S., Sharma, A., Chanana, I., Patel, S. K. S., Kulshrestha, S., & Kumar, P. (2023). Vinca alkaloids as a potential cancer therapeutics: recent update and future challenges. In 3 Biotech (Vol. 13, Number 6). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/s13205-023-03636-6.
Gamage, H. K., & Schmidt, S. (2009). Short Communication. A robust method for chromosome quantification and ploidy determination in woody species. Australian Journal of Botany, 57(2), 87–93. https://doi.org/10.1071/BT09006.
Husain, I., Surdaya, T., & Purnomo, S. H. (2022). Induksi Mutasi Menggunakan Kolkisin pada Umbi Bawang Merah (Allium ascalonicum L.) Varietas Tajuk. Jurnal Hortikultura Indonesia, 13(1), 1–7. https://doi.org/10.29244/jhi.13.1.1-7.
Jamal, Q. M. S., & Ahmad, V. (2024). Identification of Metabolites from Catharanthus roseus Leaves and Stem Extract, and In Vitro and In Silico Antibacterial Activity against Food Pathogens. Pharmaceuticals, 17(4). https://doi.org/10.3390/ph17040450.
Kusnuriyanti, E., Fatikasari, S., Fitriasari, I., & Sfofi, M. (2017). Hasil Mutasi Genetik Dengan Ekstrak Etanolik Daun Tapak Dara (Catharanthus roseus (L.) D. Don). Jurnal Wiyata, 4 no 2, 121–127.
Kwon, S.-J., Seo, D.-Y., Cho, G.-Y., Lee, M.-S., Moon, Y.-J., Boo, H.-O., Woo, S.-H., & Kim, H.-H. (2016). Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata. Korean Journal of Plant Resources, 29(3), 347–354. https://doi.org/10.7732/kjpr.2016.29.3.347.
Listiana, I. (2016). Pengaruh medan magnet 0, 2 mT terhadap pertumbuhan generatif tanaman tomat (Lycopersicum esculentum Mill.) yang diinfeksi Fusarium oxysporum [Doctoral dissertation].
Manzoor, A., Ahmad, T., Bashir, M. A., Hafiz, I. A., & Silvestri, C. (2019). Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. In Plants (Vol. 8, Number 7). MDPI AG. https://doi.org/10.3390/plants8070194.
Muhlisyah, N., Muthiadin, C., Farhatul Wahidah, B., Aziz, I. R., Biologi, J., Sains, F., Teknologi, D., Makassar, A., & Sultan, J. (2014). Preparasi Kromosom Fase Mitosis Markisa Ungu (Passiflora edulis) Varietas Edulis Sulawesi Selatan. 2(1), 48–55.
Novak, F.J., dan H Brunner. “Plant breeding: Induced mutation technology for crop improvement.” 4, no. IAIE Bulletin (1992): 25–33.
Paul, A., Acharya, K., & Chakraborty, N. (2023). Biosynthesis, extraction, detection and pharmacological attributes of vinblastine and vincristine, two important chemotherapeutic alkaloids of Catharanthus roseus (L.) G. Don: A review. In South African Journal of Botany (Vol. 161, pp. 365–376). Elsevier B.V. https://doi.org/10.1016/j.sajb.2023.08.034.
Purbosari, P. P., & Puspitasari, E. D. (2018). Pengaruh ekstrak etanol daun tapak dara (Catharanthus roseus L.) dan kolkisin terhadap perkecambahan biji cabai rawit hibrida (Capsicum annuum). BIOEDUKASI: Jurnal Pendidikan Biologi, 9(2), 181–187.
Soedjono, S. (2003). Aplikasi mutasi induksi dan variasi somaklonal dalam pemuliaan tanaman. Jurnal Litbang Pertanian, 22(2), 2003, 70-78.
Udofia, E. G., Daudu, O. Y., Isong, A. E., Odeyemi, F., Adeboye, S. E., Adeshina, D. A., Osisami, O. F., & Joseph, R. I. (2024). The Effects of Colchicine on the Mitotic Behaviour of Selected Indigenous Tomato (Solanum Lycopersicum L.) Accession. BADEGGI JOURNAL OF AGRICULTURAL RESEARCH AND ENVIRONMENT, 6(2), 80–88. https://doi.org/10.35849/bjare202402/184/008.
Wang, X., Cheng, Z. M. M., Zhi, S., & Xu, F. (2016). Breeding triploid plants: A review. In Czech Journal of Genetics and Plant Breeding (Vol. 52, Number 2, pp. 41–54). Czech Academy of Agricultural Sciences. https://doi.org/10.17221/151/2015-CJGPB.
Zulfiqar, T. (2022). Effect of Colchicine on Induction of Ploidy and Other Morphological Features of Different Crops: A Review. Annals of Advanced Biomedical Sciences, 5(1). https://doi.org/10.23880/aabsc-16000172.
?????????????, Ma, K.-B., Cho, K.-S., Jung, H.-W., Seo, H.-J., & Kang, S.-S.. (2018). ?? ??? ??? ?? ??? ?? ? ??? ?? Induction of A Chromosome-doubled Persimmon (Diospyros kaki Thunb.) by in vitro Colchicine Treatment. https://doi.org/10.7732/kjpr.2018.31.5.515.
DOI: https://doi.org/10.14421/biomedich.2026.151.1171-1176
Refbacks
- There are currently no refbacks.
Copyright (c) 2026 Tri Wulandari, Ika Listiana, Ovi Prasetya Winandari
Biology, Medicine, & Natural Product Chemistry |
|
