Malondialdehyde Levels in Wistar Rat Liver after High Fat Diet and Mango Tea Mistletoe Extract Intake

Putri Maharani Aulia Rakhman, Ari Hayati, Nour Athiroh Abdoes Sjakoer

Abstract


Malondialdehyde (MDA) levels increase due to the accumulation of free radicals that exceed normal limits, leading to oxidative stress. Mango mistletoe and tea mistletoe (MESA-DP) are known to contain secondary metabolites that function as natural antioxidants. The use of antioxidants derived from these mistletoes can serve as an alternative to reduce MDA levels and neutralize free radicals. This study aimed to determine the dose of MESA-DP extract that is more effective in reducing MDA levels. This research employed a Randomized Pre Test–Post Test Control Group design using 35 male Wistar rats (Rattus norvegicus), which were divided into five treatment groups with 4 repetition: G1 normotensive as a control without high fat diet (HFD) and without gavage; G2 as an HFD control without gavage; G3 receiving HFD and MESA-DP of 50 mg/KgBW; G4 receiving HFD and MESA-DP of 100 mg/KgBW; and G5 receiving HFD and MESA-DP of 200 mg/KgBW. Data were analyzed using a one-way analysis of variance (ANOVA) to determine differences in hepatic MDA levels among groups. The results showed significant differences in hepatic MDA. Levels among all treatment groups following administration of MESA-DP extracts. The extracts demonstrated an effect in reducing MDA levels and enhance SOD levels thanks to its antioxidant properties. The most effective doses were found in group G3 50 mg/KgBW with an MDA level of 17,604 ± 0,389.


Keywords


high fat diet; malondialdehyde; mango mistletoe; tea mistletoe; wistar rats

Full Text:

PDF

References


As, A. N. (2009). Kontraktilitas Pembuluh Darah Arteri Ekor Tikus Terpisah dengan atau tanpa Endotel setelah Pemberian Ekstrak Scurulla oortiana (Benalu Teh). Jurnal Universitas Islam Malang, 3(4), 31-34.

As, N. A., & Sulistyowati, E. (2013). Scurrula atropurpurea Increases Nitric Oxide and Decreases Malondialdehyde in Hypertensive Rats. Universa Medicina, 32(1), 44-50.

Athiroh, N., & Wahyuningsih, D. (2017). Study of Superoxide Dismutase and Malondialdehyde Concentrations in Mice after Administration of Methanolic Extract of Scurrula atropurpurea (Bl.). Jurnal Kedokteran Hewan - Indonesian Journal Of Veterinary Sciences, 11(1), 19–22. Https://Doi.Org/10.21157/j.Ked.Hewan.v11i1.5431

Athiroh, N., Permatasari, N., Sargowo, D., & Widodo, M. A. (2014). Antioxidative and Blood Pressure-Lowering Effects of Scurrula atropurpurea on Deoxycorticosterone Acetate–Salt Hypertensive Rats. Biomarkers And Genomic Medicine, 6(1), 32-36.

Curfs, H. A. J., Chwalibog, A., Savenije, B. S., dan Ritskes-Hoitinga, M. 2010. Nutrient Requirements, Experimental Design, and Feeding Schedules in Animal Experimentation. Chapter 11, p. 308.

Dita, M. C. (2021). Enzyme Linked Immunosorbent Assay (Elisa). Natural Sciences Engineering and Technology Journal, 1(2), 29–38. Https://Doi.Org/10.37275/Nasetjournal.v1i2.6

Djati, M., Naomi Azerlyn, D. R., Kusuma, K. H., Rosyadah, N., Sarah Kamila, F., Annisa, Y., Ika Christina, Y., Rizqi Dwijayanti, D., & Widodo, N. (2025). Antioxidant Properties and Quantification of Phenolic and Flavonoid Compounds in Alpinia Purpurata (Viell.) K. Schum Ethanol Extract. Biotropika: Journal Of Tropical Biology, 13(1), 11– 15

Faisal, Majida Ramadhan, Ramizard Rafsanjani, dan Terra Januarista, (2025), Optimization of Analysis Method on Total Phenol Content and Antioxidant Activity in Peel and Seeds of White Pomegranate (Punica granatum L.). Biotropika: Journal Of Tropical Biology, 13(1), 33–41. Https://Doi.Org/10.21776/Ub.Biotropika.2025.013.01.05

Lestari, R. D., Sjakoer, N. A. A., & Mubarakati, N. J. (2023). Pengaruh Ekstrak Benalu Mangga Terhadap Kadar Malondealdehid (MDA) pada Tikus Hipertensi yang Dipapar Doca-Garam. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 8(2), 135–141. Https://Doi.Org/10.33474/Ejbst.v8i2.402

Ma’Ruf, M., Sjakoer, N. A. A., Mubarakati, N. J., & Kumalasari, E. (2025). Pengaruh Pemberian Benalu Teh dan Benalu Mangga Terhadap Kadar Malondialdehid Serum pada Tikus Wistar Jantan. Pharmasipha : Pharmaceutical Journal of Islamic Pharmacy, 9(1), 1–11.

Munawwaroh, R. (2019). Efek Dekokta Daun Pulutan (Urena lobata) Terhadap Kadar Superoxide Dismutase (SOD) dan Malondialdehyde (MDA) Hepar Ikan Zebra (Danio rerio) yang Dipapar Malathion Secara Kronik. Jurnal Bio Komplementer Medicine, 6(3).

Murwani, S., Ali, M., Dan Muliartha K. 2006. Diet Aterogenik Pada Tikus Putih (Rattus novergicus Strain Wistar) Sebagai Model Hewan Aterosklerosis. Jurnal Kedokteran Brawijaya , Vol. Xxii, No. 1, p. 6-9.

Puspa Eldiaputri Kiai Demak, & Carla Felly Kairupan, Nur Anindhita Kurniawaty Wijaya. (2025). Pengaruh Pemberian Ekstrak Alga Cokelat (Sargassum polycystum) dari Perairan Pulau Nain Terhadap Gambaran Histopatologik Hepar Tikus Putih Strain Wistar Jantan (Rattus novergicus) yang Diberi Diet Tinggi Lemak. Maret 2025, 6(1).

Sengupta, P. 2013. The Laboratory Rat: Relating Its Age With Human’s. International Journal of Preventive Medicine, Vol 4, No 6.

Silvia, P. A., Sjakoer, N. A. A., Universitas Islam Malang, Mubarakati, N. J., & Universitas Islam Malang. (2024). Comparison of Antioxidant Activity of Mistletoe Mango Leaves Extract (Dendrophtoe pentandra (L.) Miq) Using Ethanol Solution and Methanol Solution Based on DPPH Method. Jsmartech, 5(1), 15–19. Https://Doi.Org/10.21776/Ub.Jsmartech.2024.005.01.15

Yeskaliyeva B, Abdull Razis Af, Modu B, Calina D and Sharifi-Rad J (2023) Oxidative Stress, Free Radicals and Antioxidants: Potential Crosstalk in The Pathophysiology of Human Diseases. Frontiers in Chemistry 11: 1158198. Doi: 10.3389/Fchem.2023.1158198

Zaric Bl, Macvanin Mt, Isenovic Er (2023) Free Radicals: Relationship to Human Diseases and Potential Therapeutic Applications. The International Journal of Biochemistry & Cell Biology 154: 106346. Doi: 10.1016/J.Biocel.2022.106346.

Zulkifli, F., Agustini, S. M., & Hasanah, A. (2016). Pengaruh Ekstrak Biji Cokelat (Theobroma cacao L) Terhadap Kadar Malondialdehid (Mda) Tikus Putih Jantan (Rattus norvegicus Strain Wistar) dengan Induksi Hiperkolesterol. Saintika Medika, 12(1), 7. Https://Doi.Org/10.22219/Sm.v12i1.5262




DOI: https://doi.org/10.14421/biomedich.2026.151.1649-1653

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2026 Putri Maharani Aulia Rakhman, Ari Hayati, Nour Athiroh Abdoes Sjakoer



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC