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Fragrant Pandan (Pandanus ammaryllifolius) Leaves Ethanol Extract as an Enhancer of Endogenous Antioxidant Defence under High-Fat Diet Conditions
Abstract
Excessive fat consumption, especially saturated and trans fatty acids, plays a significant role in metabolic disorders such as obesity, dyslipidemia, and cardiovascular disease through increased oxidative stress. Malondialdehyde (MDA), the end product of lipid peroxidation, serves as a biomarker of oxidative damage, whereas indirect bilirubin acts as an endogenous antioxidant. This study aimed to evaluate the effect of fragrant pandan leaves ethanol extract (FPLEE) in reducing MDA levels and modulating indirect bilirubin activity in rats fed a high-fat diet (HFD). The experimental method used 24 male Rattus norvegicus divided into six treatment groups: normal control (NC), positive control (C+), negative control (C-), and three FPLEE treatment groups with different doses (8, 16, and 32 mg/200 g BW/day). Administration was carried out for 14 days after 14 days of HFD induction. MDA levels were measured using the ELISA method, while total bilirubin and direct bilirubin were measured using spectrophotometry to determine indirect bilirubin levels. Statistical analysis was performed using one-way ANOVA and the post hoc Bonferroni test. The results showed that there were no significant differences in MDA levels between groups, although the C+ group fed a HFD showed a tendency toward increased MDA. Indirect bilirubin levels increased significantly in the low-dose FPLEE group (8 mg/200 g BW/day), indicating increased endogenous antioxidant activity, while higher doses did not. Thus, FPLEE has the potential to be an effective natural phytopharmaceutical agent in enhancing the body's antioxidant defence against oxidative stress caused by excessive fat consumption, especially at low doses.
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Amponsah-Offeh, M., Diaba-Nuhoho, P., Speier, S., & Morawietz, H. (2023). Oxidative Stress, Antioxidants and Hypertension. Antioxidants, 12(281), 1–22. https://doi.org/10.3390/antiox12020281
Bareetseng, S. (2022). The Worldwide Herbal Market: Trends and Opportunities. Journal of Biomedical Research & Environmental Sciences, 3(5), 575–584. https://doi.org/10.37871/jbres1482
Boriskina, O. S., Eliseeva, T. I., Tush, E. V., Soodaeva, S. K., Khramova, R. N., Khristianovich, E. A., … Kubysheva, N. I. (2024). Bronchial Asthma and Bilirubin. Opera Medica et Physiologica, 11(1), 115–122. https://doi.org/10.24412/2500-2295-2024-1-115-122
Consoli, V., Sorrenti, V., Grosso, S., & Vanella, L. (2021). Heme Oxygenase-1 Signaling and Redox Homeostasis in Physiopathological Conditions. Biomolecules, 11(589), 1–23. https://doi.org/10.3390/biom11040589
He, M., Lin, X., Zheng, Y., Liang, Q., Li, M., Qiang, W., … Shi, B. (2025). Indirect bilirubin is inversely associated with diabetic retinopathy risk and is a potential predictive biomarker. World Journal of Diabetes, 16(9), 1–12. https://doi.org/10.4239/wjd.v16.i9.110590
Irato, P., & Santovito, G. (2021). Enzymatic and Non-Enzymatic Molecules with Antioxidant Function. Antioxidants, 10(579), 1–4. https://doi.org/10.3390/antiox10040579
Jiang, Shuai; Liu, H. L. C. (2021). Dietary Regulation of Oxidative Stress in Chronic Metabolic Diseases. Foods, 10(1854), 1–18.
Jomova, K., Alomar, S. Y., Valko, R., Liska, J., Nepovimova, E., Kuca, K., & Valko, M. (2025). Flavonoids and their role in oxidative stress, inflammation, and human diseases. Chemico-Biological Interactions, 413, 1–44. https://doi.org/10.1016/j.cbi.2025.111489
Juan, C. A., de la Lastra, J. M. P., Plou, F. J., & Pérez-Lebeña, E. (2021). The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences, 22(4642), 1–21. https://doi.org/10.3390/ijms22094642
Lankin, V. Z., Tikhaze, A. K., & Melkumyants, A. M. (2023). Malondialdehyde as an Important Key Factor of Molecular Mechanisms of Vascular Wall Damage under Heart Diseases Development. International Journal of Molecular Sciences, 24(128), 1–13. https://doi.org/10.3390/ijms24010128
Lennicke, C., & Cocheme, H. M. (2021). Redox metabolism: ROS as specific molecular regulators of cell signaling and function. Molecular Cell, 81(18), 3691–3707. https://doi.org/10.1016/j.molcel.2021.08.018
Moratilla-Rivera, I., Sánchez, M., Valdés-González, J. A., & Gómez-Serranillos, M. P. (2023). Natural Products as Modulators of Nrf2 Signaling Pathway in Neuroprotection. International Journal of Molecular Sciences, 24(3748), 1–26. https://doi.org/10.3390/ijms24043748
Obeagu, E. I., & Obeagu, G. U. (2024). Antioxidants and the Prevention of Neonatal Jaundice: A Narrative Review. International Journal of Medical Sciences and Pharma Research, 10(4), 28–34. https://doi.org/10.22270/ijmspr.v10i4.120
Padhi, S., Gupta, K. K., Saraugi, S. S., Sehrawat, R., & Routray, W. (2024). Comparative analysis of non-thermal technologies and solvent systems for the extraction and characterization of phytochemicals and antioxidants in Pandanus amaryllifolius. Chemical Engineering Research and Design, 210, 212–229. https://doi.org/10.1016/j.cherd.2024.08.036
Ruscica, M., Ferri, N., Banach, M., Sirtori, C. R., & Corsini, A. (2022). Side effects of statins: from pathophysiology and epidemiology to diagnostic and therapeutic implications. Cardiovascular Research, 118(17), 3288–3304. https://doi.org/10.1093/cvr/cvac020
Sang, Y., Huang, W., Lin, J., Yang, L., Zhou, Y., Yu, C., … Kong, X. (2025). Fluorinated sulfonamide-flavonoid derivatives as novel Keap1-Nrf2 inhibitors: Potent induction of cytoprotective gene HO-1 in vivo. European Journal of Medicinal Chemistry, 291. https://doi.org/10.1016/j.ejmech.2025.117650
Sukanty, N. M. W., Ariani, F., & Yunita, L. (2024). The Effectiveness of Fragrant Pandan Leaves Ethanol Extract in Reducing Cholesterol Levels In High-Fat Diet-Induced Rats. Biology, Medicine, & Natural Product Chemistry, 13(2), 505–509. https://doi.org/10.14421/biomedich.2024.132.505-509
Xiao, W., Xiong, Y., Li, Y., Chen, Z., & Li, H. (2023). Non-Enzymatically Colorimetric Bilirubin Sensing Based on the Catalytic Structure Disruption of Gold Nanocages. Sensors, 23(2969), 1–13. https://doi.org/10.3390/s23062969
Yang, R., Wang, C., Ye, H., Gao, F., Cheng, J., Zhang, T., & Guo, M. (2019). Effects of feeding hyperlipidemia rats with symbiotic oat-based frozen yogurt on serum triglycerides and cholesterol. Food Science and Nutrition, 7(3), 1096–1103. https://doi.org/10.1002/fsn3.949
Zhang, H., Halmos, B., & Westerterp, M. (2023). Statins for ACTA2 mutation-driven atherosclerosis? Europan Heart Journal, 44(29), 2727–2729. https://doi.org/10.1093/eurheartj/ehad364
Zhu, Y., Li, D., Li, J., Wang, Y., Chen, Z., Lv, Q., … Zhang, W. (2024). Association of elevated baseline bilirubin caused by preadmission statin use with cardiovascular prognosis in patients undergoing elective percutaneous coronary intervention. Scientific Reports, 14(1), 1–10. https://doi.org/10.1038/s41598-024-71497-x
Žiberna, L., Jenko-Pražnikar, Z., & Petelin, A. (2021). Serum Bilirubin Levels in Overweight and Obese Individuals: The Importance of Anti-Inflammatory and Antioxidant Responses. Antioxidants, 10(9), 1–16. https://doi.org/10.3390/antiox10091352
DOI: https://doi.org/10.14421/biomedich.2025.142.1171-1176
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Copyright (c) 2025 Ni Made Wiasty Sukanty, Laksmi Nur Fajriani, I Putu Bayu Agus Saputra, Iptan Ariki, I Ketut Agus Yura Aditya, Angeline Devon Venansius
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