The Effectiveness of Honey Purity Testing from Several Regions in Central Sulawesi Province

I Made Budiarsa, Manap Trianto, Akram Akram, Yulia Windarsih, Abdul Ashari

Abstract


Honey is a valuable natural product with nutritional, medicinal, and economic importance, but its high commercial value makes it prone to adulteration, which threatens consumer trust and product quality. This study aimed to evaluate the effectiveness of simple purity tests in identifying adulteration in honey samples collected from several regions in Central Sulawesi Province. A total of 12 honey samples from different bee species and floral sources were analyzed using seven tests, namely solubility, cloudiness and foam, heating, hexagonal pattern, seepage, raw fish, and onion tests. The results showed that most tests successfully distinguished pure honey from adulterated samples. Specifically, the solubility, cloudiness and foam tests, as well as heating, and raw fish tests indicated that 66.67% of the samples were pure, while the hexagonal pattern, seepage, and onion tests confirmed purity in all samples (100%). These findings demonstrate that simple, low-cost methods can provide reliable initial screening of honey purity and are suitable for routine use in local communities where advanced laboratory facilities are limited. The application of these tests is expected to support honey quality assurance, protect consumer trust, and promote the sustainable development of the beekeeping industry in Central Sulawesi.

Keywords


Adulteration detection; Honey purity; Regional quality; Simple tests; Sulawesi honey

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References


Abeshu, M. A., & Geleta, B. (2016). Medicinal uses of honey. Biology and Medicine, 8(2), 1000276.

Afroz, R., Tanvir, E. M., & Hossain, M. M. (2023). Physical properties of honey. Honey: composition and health benefits, 12-31.

Al-Kafaween, M. A., Alwahsh, M., Mohd Hilmi, A. B., & Abulebdah, D. H. (2023). Physicochemical Characteristics and Bioactive Compounds of Different Types of Honey and Their Biological and Therapeutic Properties: A Comprehensive Review. In Antibiotics,12(2).

Anagaw, Y. K., Ayenew, W., Limenh, L. W., Geremew, D. T., Worku, M. C., Tessema, T. A., & Mitku, M. L. (2024). Food adulteration: Causes, risks, and detection techniques. SAGE Open Medicine, 12, 20503121241250184.

Bose, D., & Padmavati, M. (2024). Honey Authentication: A review of the issues and challenges associated with honey adulteration. Food Bioscience, 61, 105004.

Choudhary, A., Gupta, N., Hameed, F., & Choton, S. (2020). An overview of food adulteration: Concept, sources, impact, challenges and detection. International Journal of Chemical Studies, 8(1), 2564-2573.

Correa-Mosquera, A. R., Quicazán, M. C., & Zuluaga-Domínguez, C. M. (2022). Shelf-life prediction of pot-honey subjected to thermal treatments based on quality attributes at accelerated storage conditions. Food Control, 142, 109237.

Damto, T. (2021). A Review on Status of Honey Adulteration and Their Detection Techniques in Ethiopia. Journal of Nutrition & Food Sciences, 11, 180.

Dobrinas, S., Soceanu, A., Birghila, S., Birghila, C., Matei, N., Popescu, V., & Constanda, L. M. (2022). Chemical analysis and quality assessment of honey obtained from different sources. Processes, 10(12), 2554.

Fakhlaei, R., Selamat, J., Khatib, A., Razis, A. F. A., Sukor, R., Ahmad, S., & Babadi, A. A. (2020). The toxic impact of honey adulteration: A review. Foods, 9(11), 1538.

García, N. L. (2018). The current situation on the international honey market. Bee World, 95(3), 89-94.

Gela, A., Gebresilassie, A., Atikem, A., Damto, T., & Woldehawariat, Y. (2023). Physicochemical properties and botanical sources of honey from different areas of Ethiopia: an implication for quality control. Journal of Food Quality, 2023(1), 9051595.

Halko, R., Pavelek, D., & Kaykhaii, M. (2024). High performance liquid chromatography-fourier transform infrared spectroscopy coupling: a comprehensive review. Critical Reviews in Analytical Chemistry, 1-12.

Inaudi, P., Garzino, M., Abollino, O., Malandrino, M., & Giacomino, A. (2025). Honey: Inorganic Composition as Possible Marker for Botanical and Geological Assignment. Molecules, 30(7), 1466.

Kahono, S., Peggie, D., Subiyakto, S., Lamerkabel, J. S. A., & Engel, M. S. (2023). Diversity, recent distribution, and nesting behavior of giant honeybees in Indonesia and their role in natural and agricultural ecosystems. In Role of Giant Honeybees in Natural and Agricultural Systems (pp. 292-304). CRC Press.

M?da?, M. N., M?rghita?, L. A., Dezmirean, D. S., Bobi?, O., Abbas, O., Danthine, S., & Nguyen, B. K. (2020). Labeling regulations and quality control of honey origin: A review. Food Reviews International, 36(3), 215-240.

Manyi-Loh, C. E., Clarke, A. M., & Ndip, R. N. (2011). An overview of honey: Therapeutic properties and contribution in nutrition and human health. African Journal of Microbiology Research, 5(8), 844-852.

Mello dos Santos, M., Sostaric, T., Lim, L. Y., & Locher, C. (2025). Physicochemical Characteristics, Antioxidant Properties, and Identification of Bioactive Compounds in Australian Stingless Bee Honey Using High-Performance Thin-Layer Chromatography. Molecules, 30(6).

Mohamat, R. N., Noor, N. R. A., Yusof, Y. A., Sabri, S., & Zawawi, N. (2023). Differentiation of High-Fructose Corn Syrup Adulterated. Foods, 12(1670), 1–10.

Mohammed, M. E. A. (2022). Factors affecting the physicochemical properties and chemical composition of bee’s honey. Food Reviews International, 38(6), 1330-1341.

Morariu, I. D., Avasilcai, L., Vieriu, M., Lupu, V. V., Ioniuc, I., Morariu, B. A., & Trandafir, L. (2024). A comprehensive narrative review on the hazards of bee honey adulteration and contamination. Journal of Food Quality, 2024(1), 3512676.

Nunes, A., Rita, C., Bromer, J. V., Azambuja, G. B., Araújo, D. N., Moura, S., & Maraschin, M. (2024). Melissopalynological methodologies for investigating honey samples–a critical approach. Anais da Academia Brasileira de Ciências, 96(suppl 3), e20230703.

Oroian, M., Paduret, S., Amariei, S., & Gutt, G. (2016). Chemical composition and temperature influence on honey texture properties. Journal of food science and technology, 53(1), 431-440.

Ranneh, Y., Akim, A. M., Hamid, H. A., Khazaai, H., Fadel, A., Zakaria, Z. A., & Bakar, M. F. A. (2021). Honey and its nutritional and anti-inflammatory value. BMC complementary medicine and therapies, 21(1), 30.

Raweh, H. S. A., Badjah-Hadj-Ahmed, A. Y., Iqbal, J., & Alqarni, A. S. (2023). Physicochemical Composition of Local and Imported Honeys Associated with Quality Standards. Foods, 12(11), 1–16.

Saxena, S., Gautam, S., & Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry, 118(2), 391–397.

Siddiqui, S. A., Pahmeyer, M. J., Mehdizadeh, M., Nagdalian, A. A., Oboturova, N. P., & Taha, A. (2022). Consumer behavior and industry implications. In The age of clean label foods (pp. 209-247). Cham: Springer International Publishing.

Tafere, D. A. (2021). Chemical composition and uses of Honey: A Review. Journal of Food Science and Nutrition Research, 4(3), 194-201.

Tomczyk, M., Czerniecka-Kubicka, A., Mi?ek, M., Sidor, E., & D?ugan, M. (2023). Tracking of Thermal, Physicochemical, and Biological Parameters of a Long-Term Stored Honey Artificially Adulterated with Sugar Syrups. Molecules, 28(4).

Tyowua, A. T., Echendu, A. M., & Adejo, S. O. (2023). Influence of particle fluorination on the stability of honey foam. Frontiers in Soft Matter, 3(June), 1–11.

Wakjira, K., Negera, T., Zacepins, A., Kviesis, A., Komasilovs, V., Fiedler, S., & Brodschneider, R. (2021). Smart apiculture management services for developing countries—the case of SAMS project in Ethiopia and Indonesia. PeerJ Computer Science, 7, e484.

Zhang, X. H., Gu, H. W., Liu, R. J., Qing, X. D., & Nie, J. F. (2023). A comprehensive review of the current trends and recent advancements on the authenticity of honey. Food Chemistry: X, 19(July), 100850.




DOI: https://doi.org/10.14421/biomedich.2025.142.1231-1240

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Copyright (c) 2025 I Made Budiarsa, Manap Trianto, Akram, Yulia Windarsih, Abdul Ashari



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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This work is licensed under a CC BY-NC