Email this article 
Detection of The p53 Gene in Formalin Fixed Tissue Archives by Polymerase Chain Reaction (PCR) Method
Abstract
Formalin fixed tissue archives are tissue biopsy results stored in formaldehyde fixative solution for a long time. The p53 gene is one of the genes that suppresses tumor occurrence and maintains genomic stability to prevent cell mutation and plays a role in the DNA regulation cycle and controls cell proliferation. Polymerase Chain Reaction (PCR) is a nucleic acid amplification technique used to denature and denature DNA or RNA using polymerase enzymes to detect the presence of the p53 gene in formalin-fixed tissue archival samples. This study aimed to detect the presence of the p53 gene in formalin-fixed tissue samples. This study used a descriptive research method with a random sampling technique conducted in the anatomical pathology laboratory and the National College of Health Sciences molecular biology laboratory. The samples in this study consisted of 10 samples consisting of 2 normal tissues used as controls and 8 formalin-fixed tissue samples. In this study, the p53 gene was detected in all samples, both normal tissue samples and formalin-fixed tissue samples after electrophoresis and visualized according to the target of 666 bp (base pair). The presence of the p53 gene qualitatively from DNA isolates can still be detected, but the gene expression level cannot be known in this study.
Keywords
Full Text:
PDFReferences
Anam, K., Cahyadi, W., Azmi, I., Senjarini, K., & Oktarianti, R. (2021). Analisis Hasil Elektroforesis DNA dengan Image Processing Menggunakan Metode Gaussian Filter. IJEIS (Indonesian Journal of Electronics and Instrumentation Systems), 11(1), 37. https://doi.org/10.22146/ijeis.58268.
Anissa, R., Lisdiana, L., & Widyayanti, A. (2024). Optimasi Metode Nested PCR untuk Deteksi Vibrio parahaemolyticus AHPND pada Udang Vaname (Litopenaeus vannamei) . LenteraBio, 1, 1–13.
Aulia, G. R., Agriansyah, A., & Daryono, B. S. (2013). Deteksi dan Skrining Pewarisan Sifat Ketahanan Penyakit Powdery Mildew pada Generasi Backross Tanaman Melon (Cucumis melo L.) Var Tacapa. Universitas Gadjah Mada.
Daryono, B. S., & Yambise, H. C. (2018). Deteksi Gen Ketahanan Terhadap Powdery Mildew Pada Melon (Cucumis melo L. ‘Aramis’). Biogenesis: Jurnal Ilmiah Biologi, 6(2), 124. https://doi.org/10.24252/bio.v6i2.5068.
Erawati dyah, Andreani Sri Utomo, & Bari Abdul Yunus. (2017). Diagnosis Dan Terapi Tumor Muskuloskeletal (Mahyudin Ferdiansyah, Ed.; pertama).
Exzoea, H. B., & Qurrohman, M. T. (2024). Detection of Candida albicans in Urine Patients of Diabetes Melitus using Polymerase Chain Reaction (PCR) Method. Jurnal Biologi Tropis, 24(3), 298–302. https://doi.org/10.29303/jbt.v24i3.7173.
Feranisa, A. (2020). Komparasi Antara Polymerase Chain Reaction (PCR) Dan Loop Mediated Isothermal Amplification (LAMp) Dalam Diagnosis Molekuler. ODONTO Dental Journal., 3(2), 145.
Hafy, Z., Larasati, V., Puspita, R. S., S, N., M, H., A, R., & R, S. (2018). Hubungan Lama Penyimpanan Sampel Arsip Jaringan Dalam Blok Parafin Terfiksasi Formalindengan Kualitas Hasil Ekstraksidna Mitokondria Jaringan. Sriwijaya Journal Of Medicine, 1(3), 157–162. https://doi.org/10.32539/sjm.v1i3.31.
Halimah, I. S., Solihat, M. F., & Djuminar, A. (2024). Optimasi Variasi Konsentrasi Agarose dan Waktu Elektroforesis Terhadap Kualitas Pita DNA Mycobacterium tuberculosis Resistan Isoniazid. Jurnal Kesehatan Siliwanngi, 4(3).
Handayani, S., & Putri, D. (2021). Perbandingan Metode Fenol-Kloroform dan Mini-Prep CTAB Untuk Isolasi DNA Tanaman Cabai (Capsicum annum L.). Serambi Biologi, 6(2), 37–41.
Hidayati, H., Saleh, E., & Aulawi T. (2016). Identifikasi Keragaman Gen BMPR-1b (Bone Morphogenetic Protein Receptor Ib) pada Ayam Arab, Ayam Kampung dan Ayam Ras Petelur Menggunakan PCR-RFLP. Jurnal Peternakan UIN Sultan Syarif Kasim, 1(13), 1–12.
Kemenkes, R. (2023). Kanker. Kementrian Kesehatan Direktorat Jenderal Pelayanan Kesehatan.
Khehra, N., Padda, I. S., & Swift, C. J. (2024). Polymerase Chain Reaction (PCR).
Kisnanto, T., Amir, M., & Soetopo, S. (2015). EKSPRESI PROTEIN P13K DAN P53 DALAM PREDIKSI RESPON TERHADAP TINDAKAN RADIOTERAPI PADA KANKER SERVIK. https://www.researchgate.net/publication/282592026.
Lucena Aguilar, G., Sánchez-López, A. M., Barberán-Aceituno, C., Carrillo-Ávila, J. A., López-Guerrero, J. A., & Aguilar-Quesada, R. (2016). DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis. Biopreservation and Biobanking, 14(4), 264–270. https://doi.org/10.1089/bio.2015.0064.
Maliza, R., Pratiwi, L., & Perwitasari, D. (2021). Uji Kualitas DNA Darah Pada Kertas Whatman Yang Diisolasi Dengan CHELEX-100 Serta Variasi Waktu Penyimpanan . The Journal Of Muhammadiyah Medical Laboratory Technologist, 2(4).
Mollah, A., Ashan, Muh. A., & Khatimah, A. H. (2022). Uji Kualitas dan Kuantitas DNA Porang (Amorphophallus Muelleri Blume) pada Beberapa Kawasan di Sulawesi Selatan. Jurnal Agritechno, 1–7. https://doi.org/10.20956/at.v15i1.688.
Prasetya, D. Y., & Suprayitno, E. (2021). Faktor Kualitas Hidup Pasien Kanker. Care:Jurnal Ilmiah Ilmu Kesehatan, 9(2), 322–333.
Prihastuti, W. (2010). Ekspresi p53 Pada Sel HeLa Terhadap Pemberian Fraksi Aktif Pandanus conoideus Lam. Varietas Buah Kuning. . Universitas Sebelas Maret.
Rahmawanti, A., Setyowati, D., & Mukhlis, A. (2021). Histopathological of Brain, Eye, Liver, Spleen Organs of Grouper Suspected VNN in Penyambuan Village, North Lombok. Jurnal Biologi Tropis, 21(1), 140–148. https://doi.org/10.29303/jbt.v21i1.2439
Ramadhani, S., Rizky, V. A., & Siregar, S. (2024). Analysis Of The Result Of DNA (Deoxyribonucleic Acid) Electrophoresis On Vaginal Mucosal Swabs With Cervical Cancer Patients. Medistra Medical Journal (MMJ), 2(1), 20–25. https://doi.org/10.35451/mmj.v2i1.2371.
Sadia, H., Ahmad Bhinder, M., Irshad, A., Zahid, B., Ahmed, R., Ashiq, S., Malik, K., Riaz, M., Nadeem, T., Ashiq, K., & Akbar, A. (2020). Determination of expression profile of p53 gene in different grades of breast cancer tissues by real time PCR. African Health Sciences, 20(3), 1273–1282. https://doi.org/10.4314/ahs.v20i3.32.
Sulistyani, M, Huda N, Prasetyo R, & Alauhdin M. (2023). Calibration of Microplate Uv-Vis Spectrophotometer for Quality Assurance Testing of Vitamin C using Calibration Curve Method . Indonesian Journal of Chemical Science , 12(3).
Willianto, (2013). Pengaruh Paparan Formalin Dalam Berbagai Kadar Terhadap DNA Muskulus Psoas Mayor Dengan Pemeriksaan Metode PCR Lokus CSF1PO, D5S818, D13S317, D21S11. Jurnal Kedokteran Forensik Indonesia, 15(1).
Yudianto, A. (2010). Analisis DNA Tulang dan Gigi Pada Lokus Short Tandem Repeat – Combined DNA Index System (STR Codis), Y-Chromosome STRs & Mitochondrial DNA (mtDNA) Akibat Paparan Panas Suhu Tinggi. Universitas Airlangga Surabaya.
DOI: https://doi.org/10.14421/biomedich.2025.142.969-975
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Anastasia Elma Wijayanti, Fitria Diniah Janah Sayekti
Biology, Medicine, & Natural Product Chemistry |
|
