The Potential of Spirulina platensis Biostimulan as a Seed Priming Agent to Enhance the Vigor and Viability of Cucumber Seeds (Cucumis sativus)

Fitri Ayu Rahmawati, Indah Wahyu Pratiwi, Kariena Samtani, Nuning Atuillah, Reza Aris Hidayatullah, Netty Ermawati, Rahmat Ali Sya'ban, Leli Kurniasari, Moch. Rosyadi Adnan

Abstract


Spirulina platensis is a microalgae that can grow in waste media, including Sugar Mill Effluent (SME). In addition, Spirulina contains phytohormones that can be utilized to enhance seed germination. Cultivating microalgae will produce biomass that can be used for priming cucumber seeds. This research aims to determine the effect of Spirulina biomass and soaking time on the germination of cucumber seeds. In this research, a factorial Completely Randomized Design (CRD) was used, consisting of 5 levels of microalgae biomass concentration: control (untreated seeds), 0, 30, 45, and 60%, with soaking time of 3 levels: 1, 2, and 3 hours. The results demonstrated that Spirulina can grow in SME-based media, as indicated by the increase in size and density of microalgae cells.  Analysis of the seed priming test results of 45% algae biomass concentration for 1 and 3 hours showed a significant effect on the parameter of vigor index, seed growth rate, and simultaneous seed growth. In the treatment with a 60% concentration and soaking time of 1, 2, and 3 hours, significant effects were observed on the parameters of MGT, vigor index, seed growth rate, and shoot length. Even though the 45% and 60% treatments showed no significant differences in the few parameters, the priming with Spirulina platensis exhibited no decrease in cucumber seed germination, these applications indicated no decrease in seed germination. This demonstrates that priming treatment with Spirulina platensis is not toxic to seedling growth.

Keywords


Biostimulant, Cucumis sativus; Seed priming, Spirulina platensis; Vigor

Full Text:

PDF

References


Adnan, M. R., Alvianti, H. Kurniawan, N. Aini, G. Oktoval, A. M., M. A. Firadus, and M. G. Mauludy. 2024. PERKEMBANGAN bioteknologi crispr/cas9 dalam pemuliaan tanaman mentimun. 15(1):37–48.

Akgül, F. 2019. Effect of spirulina platensis ( gomont ) geitler extract on seed germination of wheat and barley. 34:148–153.

Alam, A. U., H. Ullah, S. K. Himanshu, R. Tisarum, S. Cha-um, and A. Datta. 2023. Seed priming enhances germination and morphological, physio-biochemical, and yield traits of cucumber under water-deficit stress. Journal of Soil Science and Plant Nutrition. 23(3):3961–3978.

Alling, T., C. Funk, and F. G. Gentili. 2023. Nordic microalgae produce biostimulant for the germination of tomato and barley seeds. Scientific Reports. 13(1):1–9.

Andayani, R. D., A. D. Rosanti, P. S. Agroteknologi, F. Pertanian, U. I. Kadiri, P. S. Kimia, F. Pertanian, U. I. Kadiri, S. Priming, A. Panas, S. Priming, and D. Viabilitas. 2023. Aplikasi seed priming untuk meningkatkan vigor and viabilitas benih sorgum ( sorghum bicolor l .). 16(1):35–39.

Andianingsih, N., A. Rosnaka, and S. Mubarok. 2021. PENGARUH pemberian hormon auksin and giberelin terhadap pertumbuhan tomat (solanum lycopersicum l.) var. aichi first di dataran medium. 3(1):48–56.

Arahou, F., I. Lijassi, A. Wahby, L. Rhazi, M. Arahou, and I. Wahby. 2023. Spirulina-based biostimulants for sustainable agriculture: yield improvement and market trends. BioEnergy Research. 16(3):1401–1416.

Ashari, H., E. N. Aziza, and B. Wijayanto. 2024. Kajian mutu benih mentimun baby (cucumis sativus l.) pada berbagai media tanam. Jurnal Agrisistem. 19(2):46–54.

Badu, R., S. Malla, S. Rawal, and S. Thapa. 2022. Effect of seed priming on germination and seedling parameters of cucumber (cucumis sativus l.) in lamjung, nepal. Turkish Journal of Agriculture - Food Science and Technology. 10(10):1997–2000.

Basavaraja, B. 2024. Influence of different concentrations of spirulina platensis extract on seed germination and seedling vigor of various crops influence of different concentrations of spirulina platensis extract on seed germination and seedling vigor of various crops. (February)

Chiaiese, P., G. Corrado, G. Colla, M. C. Kyriacou, and Y. Rouphael. 2018. Renewable sources of plant biostimulation: microalgae as a sustainable means to improve crop performance. Frontiers in Plant Science. 871(December):1–6.

Dharma, I. P. E. S., S. Samudin, and Adrianton. 2015. Perkecambahan benih pala (myristica fragrans houtt.) dengan metode skarifikasi and perendaman zpt alami. Jurnal Agrotekbis. 3(2):158–167.

Fadhilah, S. 2020. Pengujian daya berkecambah berdasarkan ista rules 2020. Balai Besar Pengembangan Pengujian Mutu Benih Tanaman Pangan And Hortikultura. 1–44.

García-Locascio, E., E. I. Valenzuela, and P. Cervantes-Avilés. 2024. Impact of seed priming with selenium nanoparticles on germination and seedlings growth of tomato. Scientific Reports. 14(1):1–12.

Haerani, N. and N. Nurdin. 2021. Uji efektivitas teknik biopriming dengan cendawan trichoderma pada perbaikan viabilitas benih and produksi mentimun. J. Agrotan. 7(1):42–54.

Harsono, N. A., F. M. Bayfurqon, and E. Azizah. 2021. Pengaruh periode simpan and konsentrasi ekstrak bawah merah (allium cepa l.) terhadap viabilitas and vigor benih timun apel (cucumis sp.). 7(8)

Kamaludin, A. M. R. and H. A. Holik. 2022. Artikel ulasan: kandungan senyawa kimia and aktivitas farmakologi spirulina sp. Indonesian Journal of Biological Pharmacy. 2(2):59.

Kartina, Mardhiana, and W. Karlina. 2021. VIGOR and viabilitas benih mentimun (cucumis sativus l.) dengan pemberian naocl and teknik pengeringan berbeda. 2(2):33–37.

Kolo, E. and A. Tefa. 2016. Pengaruh kondisi simpan terhadap viabilitas and vigor benih tomat (lycopersicum esculentum mill). Savana Cenanda. 1(03):112–115.

Kurnia, T. D., E. Pudjihartati, and L. T. Hasan. 2017. Bio-priming benih kedelai (glycine max (l.) merrill) untuk meningkatkan mutu perkecambahan. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati. 1(April):62–67.

Lebeharia, S. M. 2016. PERTUMBUHAN AND KUALITAS BIOMASSA Spirulina Platensis YANG DI PRODUKSI PADA MEDIA ZAROUK MODIFIKASI

Luo, Y., J. Liang, G. Zeng, M. Chen, D. Mo, G. Li, and D. Zhang. 2018. Seed germination test for toxicity evaluation of compost: its roles, problems and prospects. Waste Management. 71:109–114.

Nurwiati, W. and C. Budiman. 2023. Uji cepat vigor benih tomat (solanum lycopersicum l.) dengan metode radicle emergence. Buletin Agrohorti. 11(2):260–265.

Oktaviana, Z., S. Ashari, S. Lestari, P. Jurusan, B. Pertanian, and F. Pertanian. 2016. Pengaruh perbedaan umur masak benih terhadap hasil panen tiga varietas lokal mentimun (cucumis sativus l.). Jurnal Produksi Tanaman. 4(3):131471.

Parnidi, T. H. RS, A. R. M. Murianingrum, and Marjani. 2022. Morfologi bunga and daya kecambah benih tanaman stevia (stevia rebaudiana bertoni m). Artikel Pemakalah Paralel. 7:132–139.

Ratnaningtyas, F. R., U. Kristen, S. Wacana, U. Kristen, and S. Wacana. 2019. Pengaruh perlakuan organomatrixpriming terhadap peningkatan mutu fisiologis benih cabai ( capsicum annuum l .) the effect of organomatrixpriming treatment toward physiological quality enhancemen. Buletin Anatomi And Fisiologi. 4(1):45–54.

Ribeiro, D. M., G. T. Zanetti, M. H. M. Julião, T. E. Masetto, J. M. L. N. Gelinski, and G. G. Fonseca. 2019. Effect of different culture media on growth of chlorella sorokiniana and the influence of microalgal effluents on the germination of lettuce seeds. Journal of Applied Biology and Biotechnology. 7(1):6–10.

Rocha, I., Y. Ma, P. Souza-Alonso, M. Vosátka, H. Freitas, and R. S. Oliveira. 2019. Seed coating: a tool for delivering beneficial microbes to agricultural crops. Frontiers in Plant Science. 10

Rolin, N., A. Zamzami, and A. Qadir. 2024. Pengaruh ukuran benih terhadap mutu kecambah kacang hijau (vigna radiata l.) varietas vima 4 and vimil 1. 12(1):123–135.

Rosita, A., D. Sugiono, and E. Azizah. 2022. Invigorasi benih timun apel (cucumis melo l.) dengan kombinasi zat pengatur tumbuh naa (naphtaleine acetic acid) and ekstrak tauge selama periode pembibitan. Jurnal Ilmiah Wahana Pendidikan. 8(10):64–72.

Se?men, E. and H. Ö. Ünlü. 2023. Effects of foliar applications of commercial seaweed and spirulina platensis extracts on yield and fruit quality in pepper ( capsicum annuum l .) effects of foliar applications of commercial seaweed and spirulina platensis extracts on yield. Cogent Food & Agriculture. 9(1)

Septirosya, T., D. R. Zulmi, and S. Zulaiha. 2024. Invigorasi benih cabai merah (capsicum annuum l.) kadaluarsa melalui teknik hydropriming menggunakan air kelapa muda. Agriprima : Journal of Applied Agricultural Sciences. 8(1):71–80.

Shakuntala, N. M., K. P. Kavya, S. I. Macha, and V. Kurnalliker. 2020. Studies on standardization of water soaking duration on seed quality in cucumber ( cucumis sativus l .) seeds. 9(4):1400–1404.

Singh, S. 2014. A review on possible elicitor molecules of cyanobacteria: their role in improving plant growth and providing tolerance against biotic or abiotic stress. Journal of Applied Microbiology. 117(5):1221–1244.

Soltani, E., F. Ghaderi-Far, C. C. Baskin, and J. M. Baskin. 2015. Problems with using mean germination time to calculate rate of seed germination. Australian Journal of Botany. 63(8):631.

Susanti, E. 2014. PENGARUH osmoconditioning dengan peg ( polyethylene glycol ) 6000 terhadap viabilitas benih kenaf ( hibiscus cannabinus l.) skripsi

Syaban, R. A., Suwardi, S. Rahayu, and Indrianingsih. 2023. Keterkaitan umur panen and lama waktu curing dengan produksi and mutu benih mentimun (cucumis sativus l.) galur mth 15. Agriprima : Journal of Applied Agricultural Sciences. 7(1):86–99.

UTEX. 2019. Iron stock solution boron stock solution. Utex.Org. 30–32.

Widawati, D., G. W. Santosa, and E. Yudiati. 2022. Pengaruh pertumbuhan spirulina platensis terhadap kandungan pigmen beda salinitias. Journal of Marine Research. 11(1):61–70.

Xie, R., K. Andg, S. Kan, J. Sauve, R. Johnson, R. Jessup, and H. Qin. 2022. The effects of microalgae as a biostimulant on seed germination. 72:119–126.




DOI: https://doi.org/10.14421/biomedich.2025.141.129-141

Refbacks



Bookmark and Share


Copyright (c) 2025 Fitri Ayu Rahmawati, Indah Wahyu Pratiwi, Kariena Samtani, Nuning Atuillah, Reza Aris Hidayatullah, Netty Ermawati, Rahmat Ali Sya'ban, Leli Kurniasari, Moch. Rosyadi Adnan



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC