Exploring the Therapeutic Potential of Kalanchoe Pinnata: A Comprehensive Analysis of Bioactive Compounds and Pharmacological Activities

Dakshina Bhusal, Manila Poudel, Ashika Tamang, Sujan Dhital, Nirmal Parajuli, Timila Shrestha, Samjhana Bharati, Binita Maharjan, Jhashanath Adhikari Subin, Bishnu Prasad Marasini, Ram Lal Swagat Shrestha

Abstract


Natural products are vital in drug discovery for their bioactive compounds. This study evaluated Kalanchoe pinnata's leaf extracts for antidiabetic, antimicrobial, antioxidant, and cytotoxic properties. GC-MS identified seven components in hexane and twenty in methanol extracts. The ethyl acetate extract had high phenolic (485.17 mg GAE/g) and flavonoid (40.25 mg QE/g) contents. Methanol extract showed strong antioxidant (IC50: 293.53 µg/mL) and anti-alpha glucosidase (IC50: 195.39 µg/mL) activity. Antimicrobial tests showed varied effectiveness, while chloroform and acetone extracts exhibited notable cytotoxicity. ADMET predictions assessed absorption and blood-brain barrier penetration, highlighting K. pinnata's potential for drug development.

Keywords


ADMET; Antioxidant; Cytotoxicity; GC-MS; Phytochemical screening

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References


Arroyave, F., Montaño, D., & Lizcano, F. (2020). Diabetes mellitus is a chronic disease that can benefit from therapy with induced pluripotent stem cells. International Journal of Molecular Sciences, 21(22), 1–28. https://doi.org/10.3390/ijms21228685

Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1). https://doi.org/10.1016/j.foodchem.2005.07.042

Banu, K. S., & Cathrine, L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science, 2(4), 25–32.

Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M. H., Elsohly, M. A., & Khan, I. A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evidence-Based Complementary and Alternative Medicine, 2014. https://doi.org/10.1155/2014/253875

Dong, J., Wang, N. N., Yao, Z. J., Zhang, L., Cheng, Y., Ouyang, D., & Lu, A. P. (2018). ADMETlab : a platform for systematic ADMET evaluation based on a comprehensively collected ADMET database. Journal of Cheminformatics, 1–12. https://doi.org/10.1186/s13321-018-0283-x

Efanova, I. B., Zaitsev, S. V, Zhivotovsky, B., Ko, M., & Efendic, S. (1998). Glucose and Tolbutamide Induce Apoptosis in Pancreatic ?-Cells. The Journal of Biological Chemistry, 273(50), 33501–33507.

George, L. O., Hr, R., & Bv, S. (2019). Antidiabetic Activity of Kalanchoe Pinnata in Alloxan-Induced Diabetic Rats. Asian Journal of Pharmaceutical and Clinical Research, 12(3), 241–245. https://doi.org/10.22159/ajpcr.2019.v12i3.30160

Harvey, A. L. (2008). Natural products in drug discovery. Drug Discovery Today, 13(19–20), 894–901. https://doi.org/10.1016/j.drudis.2008.07.004

Kalemba, D., & Kunicka, A. (2005). Antibacterial and Antifungal Properties of Essential Oils. Current Medicinal Chemistry, 10(10). https://doi.org/10.2174/0929867033457719

Kimmel, B., & Inzucchi, S. E. (2005). Oral Agents for Type 2 Diabetes: An Update. Clinical Diabetes, 23(2), 64–76.

Lalau, J., & Race, J. (1999). Lactic Acidosis in Metformin Therapy. Drugs, 58, 55–60.

Nagpal, G., & Sharma, M. (2020). Kalanchoe Pinnata and Its Remedial Properties To Treat Kidney Stone– a Lesser Known Plant. International Journal of Biology, Pharmacy and Allied Sciences, 9(11). https://doi.org/10.31032/ijbpas/2020/9.11.5259

Ojewole, J. A. O. (2005). Antinociceptive , anti-inflammatory and antidiabetic effects of Bryophyllum pinnatum ( Crassulaceae ) leaf aqueous extract. Journal of Ethno-Pharmacology, 99, 13–19. https://doi.org/10.1016/j.jep.2005.01.025

Okwu, D. E., & Nnamdi, F. U. (2011). Two novel flavonoids from Bryophyllum pinnatum and their antimicrobial activity. Journal of Chemical and Pharmaceutical Research, 3(2), 1–10.

Olowa, L. F., & Nuñeza, O. M. (2013). Brine Shrimp Lethality Assay of the Ethanolic Extracts of Three Selected Species of Medicinal Plants from Iligan City , Philippines. International Research Journal of Biological Sciences, 2(11), 74–77.

Patil, S. B., Dongare, V. R., Kulkarni, C. R., Joglekar, M. M., & Arvindekar, A. U. (2013). Antidiabetic activity of Kalanchoe pinnata in streptozotocin-induced diabetic rats by glucose independent insulin secretagogue action. Pharmaceutical Biology, 1–8. https://doi.org/10.3109/13880209.2013.794364

Rajsekhar, P. B., Arvind Bharani, R. S., Ramachandran, M., Jini Angel, K., & Rajsekhar, S. P. V. (2016). The “wonder plant” Kalanchoe pinnata (linn.) pers.: A review. Journal of Applied Pharmaceutical Science, 6(3), 151–158. https://doi.org/10.7324/JAPS.2016.60326

Ramachandran, S., Vamsikrishna, M., Gowthami, K. ., Battu, H., & Dhanaraju, M. D. (2011). Assessment of Cytotoxic Activity of Agave cantula Using Brine Shrimp ( Artemia salina ) Lethality Bioassay. Asian Journal of Scientific Research, 4(1).

Sanna, D., Delogu, G., Mulas, M., Schirra, M., & Fadda, A. (2012). Determination of Free Radical Scavenging Activity of Plant Extracts Through DPPH Assay: An EPR and UV–Vis Study. Food Analytical Methods, 5(4), 759–766. https://doi.org/10.1007/s12161-011-9306-1

Singh, N. P., & Saini, V. (2009). Epidemiology of kidney stones. Journal International Medical Sciences Academy, 22(3), 129–131.

Waghulde, S., Kale, M. K., & Patil, V. (2020). Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants. Proceedings, 41(1), 47. https://doi.org/10.3390/ecsoc-23-06703

Zhang, A., Sun, H., & Wang, X. (2013). Recent advances in natural products from plants for treatment of liver diseases. European Journal of Medicinal Chemistry, 63, 570–577. https://doi.org/10.1016/j.ejmech.2012.12.062




DOI: https://doi.org/10.14421/biomedich.2024.132.617-625

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Copyright (c) 2025 Dakshina Bhusal, Manila Poudel, Ashika Tamang, Sujan Dhital, Nirmal Parajuli, Timila Shrestha, Samjhana Bharati, Binita Maharjan, Jhashanath Adhikari Subin, Bishnu Prasad Marasini, Ram Lal Swagat Shrestha



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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