Isolation and Antibacterial Activity of Sembung (Blumea balsamifera) Leaf Essential Oil L., DC

Tika Afriani, Azimatur Rahmi, Mia Darmita Sari

Abstract


Sembung leaf essential oil is a complex mixture in the form of oily yellow liquid with a unique aroma. It contains various volatile components that have antibacterial, antifungal, antioxidant and cytotoxic activities. This study aims to determine the antibacterial activity of essential oil Blumea balsamifera L., DC against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Isolation of essential oil using steam distillation method with 0.21% rendement. Inhibition activity was performed by test tube method with variation concentrations of essential oil were 10%, 20%, 40%, 80%, and 100% for each bacteria. Results of this study showed that the highest antibacterial activity on Escherichia coli at 80% concentration with an inhibition zone diameter of 27.53 mm, 100% concentration for Pseudomonas aeruginosa with an inhibition zone diameter 25.72 mm and 100% concentration for Staphylococcus aureus with inhibition zone diameter 27.31 mm. Essential oil from (Blumea balsamifera) L., DC leaves have a strong activity inhibition category against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

Keywords


Antibacterial activity; Blumea balsamifera; essential oil; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus

Full Text:

PDF

References


Boligon, A. A., Feltrin, A. C., Athayde, M. L., & Feltrin, A. C. (2013). Determination of chemical composition, antioxidant and antimicrobial properties of Guzuma ulmifolia essential oil. American Journal of Essential Oils and Natural Products, 1(1), 23-27.

Bujung, A. H., Homenta, H., & Khoman, J. A. (2017). Uji daya hambat ekstrak biji buah alpukat (Persea americana Mill.) terhadap pertumbuhan Streptococcus mutans. e-GiGi, 5(2).

Dewantari, R., Lintang, M., & Nurmiyati, N. (2018). Jenis Tumbuhan yang Digunakan sebagai Obat Tradisional Di Daerah Eks-Karesidenan Surakarta. Bioedukasi UNS, 11(2), 117-122.

Fakayode, O. A., & Abobi, K. E. (2018). Optimization of oil and pectin extraction from orange (Citrus sinensis) peels: a response surface approach. Journal of Analytical Science and Technology, 9(1), 1-16.

Hanh, T. T. H., Giang, V. H., Trung, N. Q., Van Thanh, N., Quang, T. H., & Cuong, N. X. (2021). Chemical constituents of Blumea balsamifera. Phytochemistry Letters, 43, 35-39.

He, C., Yang, P., Wang, L., Jiang, X., Zhang, W., Liang, X., ... & Lv, C. (2020). Antibacterial effect of Blumea balsamifera DC. essential oil against Haemophilus parasuis. Archives of Microbiology, 202, 2499-2508.

Jamaludin, N., Pulungan, M. H., & Warsito, W. (2017). Uji Aktivitas Antibakteri Minyak Atsiri Jeruk Purut (Citrus hystrix DC) terhadap Klebsiella pneumoniae ATCC. Industria: Jurnal Teknologi dan Manajemen Agroindustri, 6(2), 61-66.

Jiang, Z. L., Zhou, Y., Ge, W. C., & Yuan, K. (2014). Phytochemical compositions of volatile oil from Blumea balsamifera and their biological activities. Pharmacognosy Magazine, 10(39), 346.

Khan, Z. A., Siddiqui, M. F., & Park, S. (2019). Current and emerging methods of antibiotic susceptibility testing. Diagnostics, 9(2), 49.

Ngajow, M., Abidjulu, J., & Kamu, V. S. (2013). Pengaruh antibakteri ekstrak kulit batang matoa (Pometia pinnata) terhadap bakteri Staphylococcus aureus secara in vitro. Jurnal Mipa, 2(2), 128-132.

Pang, Y., Wang, D., Fan, Z., Chen, X., Yu, F., Hu, X., ... & Yuan, L. (2014). Blumea balsamifera—A phytochemical and pharmacological review. Molecules, 19(7), 9453-9477.

Putri, Septi Dwi Putri., Rusdi & Asra, Ridho. 2020. A Review: Antioxidant Activities of Sembung Leaves (Blumea Balsamifera (L.) DC). EAS Journal of Pharmacy and Pharmacology, (2),(5).

Rahardjo, S. S. (2016, April). Review tanaman sembung [Blumea balsamifera (L.)]. In Proceeding of Mulawarman Pharmaceuticals Conferences (Vol. 3, pp. 18-28).

Roger, T., Pierre-Marie, M., & Igor, V. K. (2015). Phytochemical screening and antibacterial activity of medicinal plants used to treat typhoid fever in Bamboutos division, West Cameroon. Journal of Applied Pharmaceutical Science, 5(6), 034-049.

Rompas, D. E., Runtuwene, M. R., & Koleangan, H. S. (2016). Analisis Kandungan Fitokimia dan Uji Aktivitas Antioksidan dari Tanaman Lire (Hemigraphis repanda (L) Hall F.). Jurnal MIPA, 5(1), 36-39.

Ruhimat, U. (2015). Daya hambat infusum daun sembung (Blumea balsamifera) terhadap pertumbuhan bakteri Escherichia coli dengan metode difusi cakram. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi, 13(1).

Septiani, V., Choirunnisa, A., & Syam, A. K. (2017). Uji Aktivitas Antimikroba Ekstrak Etanol Daun Karuk (Piper sarmentosum Roxb.) terhadap Streptococcus mutans dan Candida albicans. Kartika: Jurnal Ilmiah Farmasi, 5(1), 7-14.

Syamsudin, R. A. M. R., Perdana, F., & Mutiaz, F. S. (2019). Tanaman temulawak (curcuma xanthorrhiza roxb) sebagai obat tradisional. Jurnal Ilmiah Farmako Bahari, 10(1), 51-65.

Tari, M., & Nely, N. (2016). Uji aktivitas antibakteri terhadap beberapa fraksi daun sembung rambat (Mikania micrantha Kunth.) terhadap bakteri penyebab penyakit kulit. Jurnal Ilmiah Bakti Farmasi, 1(2).

Widyastuti, W., & Farizal, F. (2014). Formulasi gel minyak nilam dan uji daya hambatnya terhadap bakteri Staphylococcus aureus. Scientia, 4(2), 60-5.




DOI: https://doi.org/10.14421/biomedich.2024.132.361-365

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2024 Tika Afriani, Azimatur Rahmi, Mia Darmita Sari



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC