Leaf Extract of Kentut (Paederia foetida L.) as a Preventive Measure Against Interleukin-6 Expression in the Liver of Mice in a Sepsis Model Injected with Escherichia coli
Abstract
Kentut leaves (Paederia foetida L.) are a medicinal plant that can be used as a preventative medicine against sepsis. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. This research aims to to determine the influence and effective dosage of Kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in a sepsis model injected with E. coli. The method used was a Completely Randomized Design (CRD). The study involved 24 white male mice divided into 6 groups. Data analysis was performed using One way ANOVA. The average values of IL-6 expression in the mouse livers for each group are as follows: KN at 7.09%±0.06; K+ at 26.36%±0.02; K- at 72.60%±0.05; PI (100mg/kgBW) at 71.04%±0.04; PII (300mg/kgBW) at 62.22%±0.02; and PIII (500mg/kgBW) at 40.92%±0.01. The research results indicate an influence of kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in the sepsis model injected with E. coli, with a significance value of 0.000 or p-value < 0.005. The effective dosage of kentut leaf extract as a preventive measure against IL-6 expression is the PIII dosage of 500mg/kg BW. The anti-inflammatory mechanism in sepsis is thought to be caused by the presence of flavonoids, alkaloids, phenolic acids, and terpenoid compounds. The most likely anti-inflammatory mechanism is believed to involve flavonoids inhibiting cyclooxygenase (COX) and lipoxygenase (LOX) enzymes involved in the synthesis of inflammatory mediators such as prostaglandins and leukotrienes, which can trigger IL-6 production.
Keywords
Full Text:
PDFReferences
Clarias, L., Pada, E., & Tnf-, D. A. N. E. (2018). Hewan Model Mus musculus Yang Diinfeksi Escherichia coli Dilihat Dari Histopatologi Skripsi Sebagai Salah Satu Syarat Memperoleh Gelar Sarjana Kedokteran Hewan.
Gallily, R., Yekhtin, Z., & Hanuš, L. O. (2018). The Anti-Inflammatory Properties of Terpenoids from Cannabis. Cannabis and Cannabinoid Research, 3(1), 292. https://doi.org/10.1089/CAN.2018.0014
Ghozali, I. (2009). Aplikasi Analisis Multivariate Dengan Program SPSS (4th ed.). Universitas Diponegoro.
Ginwala, R., Bhavsar, R., Chigbu, D. G. I., Jain, P., & Khan, Z. K. (2019). Potential Role of Flavonoids in Treating Chronic Inflammatory Diseases with a Special Focus on the Anti Inflammatory Activity of Apigenin. Antioxidants, 8(2), 1–28. https://doi.org/10.3390/ANTIOX8020035
Kaukonen, K.-M., Bailey, M., Pilcher, D., Cooper, D. J., & Bellomo, R. (2015). Systemic inflammatory response syndrome criteria in defining severe sepsis. The New England Journal of Medicine, 372(17), 1629–1638. https://doi.org/10.1056/NEJMOA1415236
Kemenkes RI. (2017). Pedoman Nasional Pelayanan Kedokteran Tata Laksana Sepsis. Kementerian Kesehatan Rebublik Indonesia.
Kemenkes. (2017). Pedoman Nasional Pelayanan Kedokteran Tata Laksana Sepsis. 342, 1–14.
Lopez, C. A. V., Valencia-Espinosa, I., González-Sánchez, F. A., Sánchez-López, A. L., Garcia Amezquita, L. E., & Garcia-Varela, R. (2022). Antioxidant, Anti-Inflammatory and Cytotoxic Activity of Phenolic Compound Family Extracted from Raspberries (Rubus idaeus): A General Review. Antioxidants 2022, Vol. 11, Page 1192, 11(6), 1199. https://doi.org/10.3390/ANTIOX11061192
Maleki, S. J., Crespo, J. F., & Cabanillas, B. (2019). Anti-inflammatory effects of flavonoids. Food Chemistry, 299(160), 1145–1156. https://doi.org/10.1016/J.FOODCHEM.2019.125124
Rosanti, D. (2016). Morfologi Tumbuhan. Erlangga.
Salamah, K. Z., & Halim, G. (2021). Uji Efektivitas Antidiare Ekstrak Etanol 96 % Daun Sembukan (Paederia foetida L .) Pada Mencit ( Mus musculus ) Yang Diinduksi Bakteri Escherichia coli. 6(2), 10–19.
Savitri, L., & Kasimo, E. R. (2022). Efek Preventif Ekstrak Daun Kentut (Paederia foetida L.) Terhadap Kadar Interleukin-6 Pada Mencit Model Sepsis Yang Diinduksi Escherichia coli. Indonesian Journal of Natural Science Education, 5(2), 38–43. https://doi.org/10.31002/NSE.V5I2.2778
Silaban, H. (2021). The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria. Journal of Drug Delivery and Therapeutics, 11(6), 61–67. https://doi.org/10.22270/JDDT.V11I6.5037
Singer, M., Deutschman, C. S., Seymour, C., & Shankar-Hari, M. (2016). The Third Internasional Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA, 315(8), 810. https://doi.org/10.1001/JAMA.2016.0287
Tanaka, T., Narazaki, M., & Kishimoto, T. (2014). IL-6 in Inflammation, Immunity, and Disease. Cold Spring Harbor Perspectives in Biology, 6(10), 16295–16296. https://doi.org/10.1101/CSHPERSPECT.A016295
Tischendorf, J. J. W., Yagmur, E., Scholten, D., Vidacek, D., Koch, A., Winograd, R., Gressner, A. M., Trautwein, C., Wasmuth, H. E., & Lammert, F. (2007). The interleukin-6 (IL6)-174 G/C promoter genotype is associated with the presence of septic shock and the ex vivo secretion of IL6. International Journal of Immunogenetics, 34(6), 413–418. https://doi.org/10.1111/J.1744-313X.2007.00712.X
Turnip, R. S., Bahri, N. F., Irmawati, & Mashudi. (2022). Peran Perawat pada Pasien Sepsis di Unit Critical Care: A Narrative Review Regina. 13(3), 18–23.
Verdure, J., Buchori, M., & Aminyoto, M. (2021). Faktor Yang Berhubungan Dengan Sepsis Pada Samarinda. 3(2), 27–37.
WHO. (2021). Sepsis. World Health Organization. https://www.who.int/news-room/factsheets/detail/sepsis
Yolandra, A. (2013). Pengaruh Ekstrak Metanol Daun Kelor (Moringa Oleifera) Terhadap Ekspresi Il-6 Jaringan Hepar Tikus Wistar (Rattus Norvegicus) Model Karsinoma Hepatoseluler Dengan Induksi Dmba (7,12-dimethylbenz(a)anthracene).
Syahruna, P. R., Mustika, A., & Faizi, M. (2020). Efek Ekstrak Kulit Manggis (Garcinia mangostana) Terhadap Murine Sepsis Score (MSS) Mencit Sepsis yang Diinduksi Shigella dysenteriae. Jurnal Medik Veteriner, 3(1), 95–100. https://doi.org/10.20473/JMV.VOL3.ISS1.2020.95-100
Dewi, I. D. A. D. ., Astuti, K. ., & Warditiani, N. . (2014). Identifikasi Kandungan Kimia Ekstrak Kulit Buah Manggis (Garcinia mangostana L.). Jurnal Farmasi Undayana, 2(4), 13–18.
Das, K., Dang, R., Sivaraman, G., & Ellath, R. P. (2018). Phytochemical Screening for Various Secondary Metabolites, Antioxidant, and Anthelmintic Activity of Coscinium fenestratum Fruit Pulp: A New Biosource for Novel Drug Discovery. Turkish Journal of Pharmaceutical Sciences, 15(2), 156. https://doi.org/10.4274/TJPS.54376
Roberts, J. A., Alobaid, A. S., Wallis, S. C., Perner, A., Lipman, J., & Sjövall, F. (2019). Defining optimal dosing of ciprofloxacin in patients with septic shock. The Journal of Antimicrobial Chemotherapy, 74(6), 1662–1669. https://doi.org/10.1093/JAC/DKZ069
Thai, T., Salisbury, B. H., & Zito, P. M. (2023). Ciprofloxacin. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK535454/
DOI: https://doi.org/10.14421/biomedich.2024.132.329-336
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Lisa Savitri, Maria Do Carmo Da Costa Freitas, Elfred Rinaldo Kasimo, Rochmad Krissanjaya, Syntia Tanu Juwita
Biology, Medicine, & Natural Product Chemistry |