The Study of Biosurfactant Stability and The Effect on Lipase Activity

Mieke Alvionita, Rukman Hertadi, Rahmat Rizki Fazli, Andi Alfira Ratna Faradisa Dewi, Tasyang Oktavia Rose


Lipase is one of hydrolase enzyme that catalyzed hydrolysis reaction of triacylglycerols into free fatty acids and monoglycerides or glycerol. These biocatalysts are widely used in several industries, namely food and pharmaceutical industry. The activity of lipase can increase significantly if the substrate forms an emulsion. Since biosurfactant has been known to have emulsification characteristic, the effect of biosurfactant addition into lipase is necessary to be investigated. It is the first report that evaluate the effect of microbial surfactant on lipase activity. The purpose of this research is to analyze the stability of biosurfactant emulsion under various conditions, such as salinity and pH as well as their potential to enhance lipase activity. Biosurfactant used was Halomonas elongata BK-AG18 from the collection of Biochemistry Research Group ITB, Bandung, Indonesia. It was found that after the addition of NaCl, there was no significant decrease in the emulsification activity of the biosurfactant. The emulsification index (IE24) of biosurfactant with several NaCl concentrations (2, 5, 10, 15, 20, and 25%) was obtained around 50%. Stability test of biosurfactant at pH range 4-10 showed the highest IE24 of biosurfactant was obtained at pH 6. The effect of biosurfactants on lipase hydrolysis activity is also discussed in this article. Lipase hydrolysis activity was tested using p-nitrophenyl palmitate substrate. The highest lipase activity was obtained after the addition of 70% biosurfactant (v/v) at 0.026 units. This study shows that biosurfactant from H. elongata BK-AG18 has the potential to increase lipase activity.


Biosurfactans; Bledug Kuwu; H. elongata; Lipase activity; Stability

Full Text:



Ahmad, Z. et al. (2021). Production, functional stability, and effect of rhamnolipid biosurfactant from Klebsiella sp. on phenanthrene degradation in various medium systems. Ecotoxicology and Environmental Safety. 207. p. 111514. DOI:10.1016/j.ecoenv.2020.111514.

Alvionita, M. and Hertadi, R. (2019). Bioconversion of Glycerol to Biosurfactant by Halophilic Bacteria Halomonas elongata BK-AG18. Indonesian Journal of Chemistry. 19(1). p. 48. DOI:10.22146/ijc.26737.

Bognolo, G. (1999). Biosurfactants as emulsifying agents for hydrocarbons. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 152. 41–52 DOI:10.1016/S0927-7757(98)00684-0

Carvalho-Gonçalves, L.C.T. de and Gorlach-Lira, K. (2018). Lipases and biosurfactants production by the newly isolated Burkholderia sp. Brazilian Journal of Biological Sciences, 5(9), pp. 57–68. DOI:10.21472/bjbs.050906.

Delorme, V., Dhouib, R., Canaan, S., Fotiadu, F., Carrière, F., & Cavalier, J. F. (2011). Effects of surfactants on lipase structure, activity, and inhibition. Pharmaceutical research, 28(8), 1831–1842. DOI:10.1007/s11095-010-0362-9

Fukaya, K. et al. (2016). Practical synthesis of the C-ring precursor of paclitaxel from 3-methoxytoluene. The Journal of Antibiotics. 69(4). pp. 273–279. DOI:10.1038/ja.2016.6.

Markande, A.R., Patel, D. and Varjani, S. (2021). A review on biosurfactants: properties, applications and current developments. Bioresource Technology. 330. p. 124963. DOI:10.1016/j.biortech.2021.124963.

Melani, N.B., Tambourgi, E.B. and Silveira, E. (2020). Lipases: From Production to Applications. Separation & Purification Reviews. 49(2). pp. 143–158. DOI:10.1080/15422119.2018.1564328.

Nor Syafrah Zambry, et al. (2017). Production and partial characterization of biosurfactant produced by Streptomyces sp. R1. Bioprocess Biosyst Eng 40:1007–1016 DOI:10.1007/s00449-017-1764-4

Oliveira, E.M. de et al. (2021). Isolation and Characterization of Biosurfactant-Producing Bacteria from Amapaense Amazon Soils. International Journal of Microbiology. Edited by Z. Li. pp. 1–11. DOI:10.1155/2021/9959550.

Purwasena, I.A. et al. (2019). Stability test of biosurfactant produced by Bacillus licheniformis DS1 using experimental design and its application for MEOR. Journal of Petroleum Science and Engineering. 183, p. 106383. DOI:10.1016/j.petrol.2019.106383.

Rahayu, Y.P., Suryanto, D. and Munir, E. (2019). Assay of Lipase and Biosurfactant Production Activity of Two Keratinolytic Bacteria Aeromonas media LU04 and Enterobacter tabaci PK09. International Journal of ChemTech Research. 12(01). pp. 121–128. DOI:10.20902/IJCTR.2019.120114.

Roy, A. (2017). A Review on the Biosurfactants: Properties, Types and its Applications. 8(1), p. 5.

Rashmi Rekha Saikia, et al. (2012). Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production. , 62(2), 753–763. DOI:10.1007/s13213-011-0315-5

Santos, D. et al. (2016). Biosurfactants: Multifunctional Biomolecules of the 21st Century. International Journal of Molecular Sciences, 17(3), p. 401. DOI:10.3390/ijms17030401.

Sharma, R. and Oberoi, H.S. (2017). Biosurfactant-Aided Bioprocessing: Industrial Applications and Environmental Impact, in Shukla, P. (ed.) Recent advances in Applied Microbiology. Singapore: Springer Singapore, pp. 55–88. DOI:10.1007/978-981-10-5275-0_3.

Silveira, E.A., Tardioli, P.W. and Farinas, C.S. (2016). Valorization of Palm Oil Industrial Waste as Feedstock for Lipase Production’, Applied Biochemistry and Biotechnology. 179(4). pp. 558–571. DOI:10.1007/s12010-016-2013-z.

Sperb, J.G.C. et al. (2018). Simultaneous Production Of Biosurfactants And Lipases From Aspergillus Niger And Optimization By Response Surface Methodology And Desirability Functions. Brazilian Journal of Chemical Engineering, 35(3), pp. 857–868. DOI:10.1590/0104-6632.20180353s20160400.

Ulisses M. F. de Oliveira, et al. (2017). Effect of the Presence of Surfactants and Immobilization Conditions on Catalysts’ Properties of Rhizomucor miehei Lipase onto Chitosan. Appl Biochem Biotechnol 184:1263–1285 DOI 10.1007/s12010-017-2622-1

Xi, W., Ping, Y. and Alikhani, M.A. (2021). A Review on Biosurfactant Applications in the Petroleum Industry’, International Journal of Chemical Engineering. Edited by A. Baghban, 2021, pp. 1–10. DOI:10.1155/2021/5477185.

Yadav, R.P. et al. (2017). Pancreatic Lipase Inhibitors from Plant Sources for Possible use as Antiobesity Drugs’, MGM Journal of Medical Sciences, 4(4), pp. 177–184. DOI:10.5005/jp-journals-10036-1166.

Zarinviarsagh, M., Ebrahimipour, G. and Sadeghi, H. (2017). Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application. Lipids in Health and Disease. 16(1), p. 177. DOI:10.1186/s12944-017-0565-8



  • There are currently no refbacks.

Copyright (c) 2024 Mieke Alvionita, Rukman Hertadi, Rahmat Rizki Fazli, Andi Alfira Ratna Faradisa Dewi, Tasyang Oktavia Rose

Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

This work is licensed under a CC BY-NC