The Protective Effect of Kentut Leaf Extract (Paederia foetida L.) on Gastric Histopathology in Escherichia coli-Infected Sepsis Mice Model

Lisa Savitri, Elfred Rinaldo Kasimo


Sepsis, a severe medical condition, signifies the systemic immune response to infection, often leading to organ dysfunction and mortality. Escherichia coli is a significant contributor to sepsis cases, particularly in gastrointestinal disorders. This study aimed to investigate the histopathological changes in the gastric tissue of mice induced by Escherichia coli infection and evaluate the protective effects of kentut leaf extract (Paederia foetida L.). Histopathological analysis revealed distinct alterations in the gastric tissue among different treatment groups. While normal mouse treatment showed no significant changes, negative control (K-) and positive control (K+) groups exhibited inflammation and hyperemia of the gastric mucosa, characterized by necrosis, degeneration, and inflammatory cell infiltration. Treatment with kentut leaf extract (P1, P2, and P3) resulted in milder inflammation compared to controls, indicating a protective effect against gastric mucosal damage induced by Escherichia coli infection. This protective mechanism is attributed to the anti-inflammatory properties of saponins, flavonoids, and essential oils present in kentut leaf extract. These findings underscore the potential therapeutic benefits of kentut leaf extract in mitigating gastric mucosal injury associated with bacterial infection.


protective effect; kentut leaf extract; gastric histopathology; Escherichia coli; sepsis

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Abdullah. (2008). Ilmu Penyakit Dalam (Internal Medical Disease). Medan: Universitas Sumatera Utara.Stickel F. 1997. Effect of Vitamin E Supplementation on Prostaglandin Concentrations in Aspirin–Induced Acute Gastric Injurying Aged Rats. Am J Clin Nutr, 66: 1218-1223.

Abriyanto, AE., Sabikis, Sudarso. (2012). Aktivitas Antifungi Ekstrak Etanol Daun Sembukan (Paedaria foetida L) terhadap Candida albicans. Pharmacy, Vol 9 No 3, Desember 2012

Angus, D.C.; van der Poll, T. (2013). Severe Sepsis and Septic Shock. N. Engl. J. Med. 2013, 369, 840–851.

Belousoviene, E.; Kiudulaite, I.; Pilvinis, V.; Pranskunas, A. (2021). Links between Endothelial Glycocalyx Changes and Microcirculatory Parameters in Septic Patients. Life 2021, 11, 790.

Bhattacharjee, P., D.P. Edelson, and M.M. Churpek. (2017). Identifying patients with sepsis on the hospital wards. Chest 151: 898–907.

Chanda, S., Deb, L., Tiwari, R. K., Singh, K., Ahmad, S. (2015). Gastroprotective mechanism of Paederia foetida Linn. (Rubiaceae) – a popular edible plant used by the tribal community of North-East India. BMC Complement. Altern. Med. 15, article number: 304.

Chung, Y.C. (2021). Anti-melanogenic effects of Paederia foetida L. extract via MAPK signaling-mediated MITF downregulations. Cosmetics 8, article number: 22.

Gosal F, Paringkoan B, Wenas NT. (2012). Patofisiologi dan Penanganan Gastropati Obat Antiiflamasi Nonsteroid. Jurnal Indonesia Medical Assoc, 62(11): 444-449.

Gyawali, B., Ramakrishna, K., & Dhamoon, A. S. (2019). Sepsis: The evolution in definition, pathophysiology, and management. SAGE open medicine, 7, 2050312119835043.

Ince, C. (2005). The microcirculation is the motor of sepsis. Crit. Care 2005, 9 (Suppl. 4), S13–S19.

Kaukonen K-M, Bailey M, Pilcher D, Cooper DJ, Bellomo R. (2015) Systemic inflammatory response syndrome criteria in defining severe sepsis. N Engl J Med 2015;372(17):1629–38.

Kumari, Preeti, Chandrawati Kumari, and Poornima Shekhar Singh. (2017) "Phytochemical screening of selected medicinal plants for secondary metabolites." Int. J. Life. Sci. Scienti. Res 2017; 3(4):1151-1157.

Lawn JE, Bianchi-Jassir F, Russell NJ, Kohli-Lynch M, Tann CJ, Hall J, et al. (2017) Group B Streptococcal Disease Worldwide for Pregnant Women, Stillbirths, and Children: Why, What, and How to Undertake Estimates? Clin Infect Dis. 2017;65(suppl_2):S89-S99.

Le, J., and J. Vilcek. (1987). Tumor necrosis factor and interleukin-1: cytokineswith multiple overlapping biological activities. Lab Invest. 56: 234.

Mora-Rillo M, Fernández-Romero N, Navarro-San Francisco C, Díez-Sebastián J, Romero-Gómez MP, Fernández FA, et al. (2015) Impact of virulence genes on sepsis severity and survival in Escherichia coli bacteremia. Virulence. 2015; (1):93-100.

Piechota, M.; Banach, M.; Irzmanski, R.; Barylski, M.; Piechota-Urbanska, M.; Kowalski, J.; Pawlicki, L. (2007). Plasma endothelin-1 levels in septic patients. J. Intensive Care Med. 2007, 22, 232–239.

Ramadhan, A., Wardani, A.K., Dlamini, D.S., Chang, C.I. (2021). Anthraquinone derivatives and its antibacterial properties from Paederia foetida stems. Nat. Prod. J.11, 193-199. 23.

Saifudin, Azis. (2014) Senyawa alam metabolit sekunder teori, konsep, dan teknik pemurnian. Deepublish, 2014.

Santi DA. (2013). Efek Jus Buah Jambu Biji (Psidium guajava linn) terhadap Gangguan Toleransi Glukosa pada Tikus Putih Jantan (Rattus norvegicus) Akibat Efek Samping Deksametason. Calyptra: Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2: 1-19.

Savitri, L., Sandhika, W., & Wahyu Widodo, A. D. (2019). Perbedaan Ekspresi Caspase 3 pada Limpa Tikus Model Sepsis yang Diinfeksi Escherichia coli ESBL dan dengan yang Diinfeksi Klebsiella pneumoniae Carbapenemase. Prosiding Seminar Nasional Multidisiplin, 2(1), 300–306. Retrieved from php/snami/article/view/705

Savitri, L. & Ihsan, K. (2020). Aktivitas Triterpenoid Kulit Batang Waru Jawa (Hibiscus tiliaceus L.) terhadap Bakteri Escherichia coli. Jurnal Kesehatan Perintis (Perintis’s Health Journal), 7 (1) 2020: 41-50.

Savitri, Lisa; Maslikah, Siti & Susilowati,. (2020). Effect of red betel leaf extract (Piper crocatum) against interleukin-1 beta (IL-1β) levels and thickness of feet oedema in Mus musculus (swiss strain) rheumatoid arthritis model. AIP Conference Proceedings. 2231. 040012. 10.1063/5.0002562.

Savitri, Lisa. (2022). Perbandingan Tingkat Keganasan Bakteri Berdasarkan Lama Waktu Kematian pada Tikus Model Sepsis yang Diinfeksi Escherichia coli ESBL dan Klebsiella pneumoniae Carbapenemase. Quagga: Jurnal Pendidikan dan Biologi. 14. 67-72. 10.25134/quagga.v14i1.5065.

Savitri, L., Kasimo, E.R. (2022). Efek Preventif Ekstrak Daun Kentut (Paederia foetida L.) Terhadap Kadar Interleukin-6 Pada Mencit Model Sepsis yang Diinduksi Escherichia coli. Indonesian Journal of Natural Science Education, 5(2): 38-43. DOI:

Savitri, L., Kasimo, E.R., Krissanjaya, R. (2023). Rahasia Daun Kentut: Peran Profilaksis Pencegahan Sepsis. Nganjuk: CV. Dewa Publishing.

Savitri, L., Tiyas, E.W., Freitas, M.D., Retnowati, A. (2023). Daun Kentut: Antibakteri Super dan Perlindungan Imun. Nganjuk: CV. Dewa Publishing.

Silaban H. (2021) The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria. JDDT. 11(6):61-7.

Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, Bellomo R, Bernard GR, Chiche JD, Coopersmith CM, Hotchkiss RS, Levy MM, Marshall JC, Martin GS, Opal SM, Rubenfeld GD, van der Poll T, Vincent JL, Angus DC. (2016) The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016 Feb 23;315(8):801-10.

Soni, R.K., Irchhaiya, R., Dixit, V., Shashi, A. (2013). Paederia foetida Linn: Phytochemistry, pharmacological and traditional uses. Int. J. Pharm. Sci. Res.4, 4525-4530.

Stoll BJ, Hansen NI, Sanchez PJ, Faix RG, Poindexter BB, Van Meurs KP, et al. (2011) Early onset neonatal sepsis: the burden of group B Streptococcal and E. coli disease continues. Pediatrics. 2011;127(5):817-26.

Trung, N.Q., Thong, N.M., Cuong, D.M., Manh, T.D., Hoang, L.P., Hien, N.K., Nam, P.C., Quang, D.T., Mechler, A., Vo, Q.V. (2021). Radical scavenging activity of natural anthraquinones: A theoretical insight. ACS Omega 6, 13391-13397.

Vakkalanka, J.P.; Harland, K.K.; Swanson, M.B.; Mohr, N.M. (2018). Clinical and epidemiological variability in severe sepsis: An ecological study. J. Epidemiol. Community Health 2018, 72, 741–745.

Wolinsky, H. (1980). A proposal linking clearance of circulating lipoproteins to tissue metabolic activity as a basis for understanding atherogenesis. Circ. Res. 1980, 47, 301–311.

Wang, L., Jiang, Y., Han, T., Zheng, C., Qin, L. (2014). A phytochemical, pharmacological and clinical profile of Paederia foetida and P. scandens. Nat. Prod. Commun. 9, 879-886.



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Copyright (c) 2024 Lisa Savitri, Elfred Rinaldo Kasimo

Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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