Antibacterial Bioactivity Test of Bilimbi Fruit Ethanol Extract (Averrhoa bilimbi Linn). Against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus

Reza Anindita, Dede Dwi Nathalia, Melania Perwitasari, Intan Kurnia Putri, Maya Uzia Beandrade, Nofria Rizki Amalia Harahap

Abstract


One of the causes of acnes is the bacteria Propionibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus. An alternative solution to this problem is to develop natural medicines from plants, one of which is the star fruit (Averrhoa bilimbi Linn). The purpose of this study was to test the bioactivity of star fruit against the growth of P. acnes, S. epidermidis, and S. aureus. This research method used an experimental research design with the treatment of A. bilimbi fruit ethanol extract concentrations of 50%, 60%, 70%, 80%, and 90%, chloramphenicol antibiotics as control (+), and sterile distilled water as control (-). All treatments were given to P. acnes, S. epidermidis, and S. aureus bacteria using the Kirby Baeur (disc diffusion) method. The results of this study were the effectiveness of the bioactivity compound of the ethanolic extract of A. bilimbi fruit seen on P. acnes and S. epidermidis bacteria with concentrations of 70%, 80%, and 90%. Thus, it can be concluded that A. bilimbi fruit has the potential to be developed as a natural medicine for acne caused by P. acnes and S. epidermidis.


Keywords


A. bilimbi fruit; Propionibacterium acnes; Staphylococcus epidermidis; Staphylococcus aureus; Kirby Bauer

Full Text:

PDF

References


Abhinitha, P., Gengatharan, R. S., Zulkiflee, S., Ganisan, P., Arumugam, S., & Kumar, N. (2019). Acne and One‘s Self Confidence: Cross-Sectional Study on Malaysian Student Population. Bangladesh Journal of Medical Science, 18(1), 83–86. https://doi.org/10.3329/bjms.v18i1.39555

Abraham, C. M. (2016). Antibacterial Effects of Averrhoa bilimbi L . Fruit Extracts. International Research Journal of Biological Sciences, 5(8), 72–74.

Afifi, R., Erlin, E., & Rachmawati, J. (2018). Antibacteria of Leaf Averrhoa bilimbi L. Towards Inhibition Of Propionibacterium acnes In-Vitro. Quagga : Jurnal Pendidikan Dan Biologi, 10(01), 10. https://doi.org/10.25134/quagga.v10i01.803

Aisyah, L. S., Jasmansyah, J., Purbaya, S., & Resnawati, T. (2019). Isolation and Antibacterial Activity of Phenol Compounds of Ethyl Acetic Extract of Red Zinger (Zingiber officinale Roscoe var. sunti). Jurnal Kartika Kimia, 2(1), 44–50. https://doi.org/10.26874/jkk.v2i1.23

Anindita, R., Yolanda, H., & Inggraini, M. (2022). Skrining Fitokimia dan Uji Antibakteri Senyawa Ekstrak Etanol Kulit Jeruk Lemon (Citrus limon (L.) Osbeck) Terhadap Staphylococcus aureus. Jurnal Bioshell, 11(2), 100–112. https://doi.org/https://doi.org/10.56013/bio.v11i2.1644

Chau, T. P., Muthusamy, M., Chinnathambi, A., Alahmadi, T. A., & Kuppusamy, S. (2021). Optimization of extraction and quantification of Flavonoids from Averrhoa bilimbi fruits using RP-HPLC and its correlation between total flavonoid content against antimicrobial activity. Applied Nanoscience (Switzerland), 13(2), 1293–1300. https://doi.org/10.1007/s13204-021-02020-1

Cheong, N. D. H., Ibrahim, F. S., & Yusof, H. (2022). Qualitative Phytochemical Screening and Antibacterial Effect of Averrhoa bilimbi Fruit Extracts Against Selected Bacteria. Malaysian Journal of Medicine and Health Sciences, 18(8), 14–20. https://doi.org/10.47836/mjmhs18.s15.3

Clinical and Laboratory Standard Insitute (CLSI). (2020). CLSI M100-ED29: 2021 Performance Standards for Antimicrobial Susceptibility Testing, 30th Edition. In Clsi (Vol. 40, Issue 1).

Cushnie, T. P. T., & Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5), 343–356. https://doi.org/10.1016/j.ijantimicag.2005.09.002

Das, S., Sultana, S., Roy, S., & Hasan, S. S. (2011). Antibacterial and cytotoxic activities of methanolic extracts of leaf and fruit parts of the plant Averrhoa bilimbi (Oxalidaceae). American Journal of Scientific and Industrial Research, 2(4), 531–536. https://doi.org/10.5251/ajsir.2011.2.4.531.536

Dinos, G. P., Athanassopoulos, C. M., Missiri, D. A., Giannopoulou, P. C., Vlachogiannis, I. A., Papadopoulos, G. E., Papaioannou, D., & Kalpaxis, D. L. (2016). Chloramphenicol derivatives as antibacterial and anticancer agents: Historical problems and current solutions. Antibiotics, 5(2), 1–21. https://doi.org/10.3390/antibiotics5020020

Drago, L. (2019). Chloramphenicol resurrected: A journey from antibiotic resistance in eye infections to biofilm and ocular microbiota. Microorganisms, 7(9), 1–12. https://doi.org/10.3390/microorganisms7090278

Farhadi, F., Khameneh, B., Iranshahi, M., & Iranshahy, M. (2019). Antibacterial activity of flavonoids and their structure–activity relationship: An update review. Phytotherapy Research, 33(1), 13–40. https://doi.org/10.1002/ptr.6208

Gallitano, S. M., & Berson, D. S. (2018). How Acne Bumps Cause the Blues: The Influence of Acne Vulgaris on Self-Esteem. International Journal of Women’s Dermatology, 4(1), 12–17. https://doi.org/10.1016/j.ijwd.2017.10.004

Garg, M., Chaudhary, S. K., Kumari, S., & Goyal, A. (2022). Phytochemical, Biological and Traditional Claims on Averrhoa bilimbi: An Overview. Indian Journal of Pharmaceutical Sciences, 84(3), 532–542. https://doi.org/10.36468/pharmaceutical-sciences.947

Hombach, M., Zbinden, R., & Böttger, E. C. (2013). Standardisation of disk diffusion results for antibiotic susceptibility testing using the sirscan automated zone reader. BMC Microbiology, 13(1). https://doi.org/10.1186/1471-2180-13-225

Kaczmarek, B. (2020). Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials—A Minireview. Materials, 13(3224), 1–13. https://doi.org/10.3390/ma13143224

Kusuma, S. A. F., Gunawan, E., & Saptarini, N. M. (2020). Antibacterial Activity of Averrhoa bilimbi Leaf Extract Against Staphylococcus Epidermidis and Staphylococcus Aureus. World Journal of Pharmaceutical Research, 9(8), 69–75. https://doi.org/10.20959/wjpr20208-18166

Legiawati, L., Halim, P. A., Fitriani, M., Hikmahrachim, H. G., & Lim, H. W. (2023). Microbiomes in Acne Vulgaris and Their Susceptibility to Antibiotics in Indonesia: A Systematic Review and Meta-Analysis. Antibiotics, 12(1), 145. https://doi.org/10.3390/antibiotics12010145

Leliqia, N. P. E., & Safitri, I. (2021). A Review of Phytochemical Properties, Antibacterial Activity, And Toxicity Study of Averrhoa bilimbi Leaves And Fruit. Journal of Pharmaceutical Science and Application, 3(1), 32. https://doi.org/10.24843/jpsa.2021.v03.i01.p04

Lisnawati, N., Kumala, S., & Rahmat, D. (2019). Formulation and evaluation of antibacterial activity of nanoparticles ointment preparation using Bilimbi extract. Pharmaceutical and Biomedical Research, 4(Atcc 25923), 32–39. https://doi.org/10.18502/pbr.v4i3.542

Mabhiza, D., Chitemerere, T., & Mukanganyama, S. (2016). Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. International Journal of Medicinal Chemistry, 2016, 1–7. https://doi.org/10.1155/2016/6304163

Masud Rana, S. M., Billah, M. M., Hossain, M. S., Saifuddin, A. K. M., Azizul Islam, S. K. M., Banik, S., Naim, Z., & Raju, G. S. (2014). Susceptibility of microorganism to selected medicinal plants in Bangladesh. Asian Pacific Journal of Tropical Biomedicine, 4(11), 911–917. https://doi.org/10.12980/APJTB.4.201414B362

Mokhtar, S. I., & Abd Aziz, N. A. (2016a). Antimicrobial Properties of Averrhoa bilimbi Extracts at Different Maturity Stages. Journal of Medical Microbiology & Diagnosis, 5(3), 3–6. https://doi.org/10.4172/2161-0703.1000233

Nathania, M., Wasito, E. B., & Hasanathuludhiyah, N. (2023). In Vitro Antibacterial Activity of Averrhoa bilimbi Leaves Ethanol Extract Against Salmonella typhi. JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga, 14(1), 43–47. https://doi.org/10.20473/juxta.v14i12023.43-47

Nurzaman, F., Djajadisastra, J., & Elya, B. (2018). Identification of Saponin Content in Red Frangipani (Plumeria rubra L.) Extract and Surfactant Potency in Cosmetic Preparations. Jurnal Kefarmasian Indonesia, 8(2), 85–93. https://doi.org/10.22435/jki.v8i2.325

Oliveira, D. A., Trento, M. V. C., Cesar, P. H. S., Braga, M. A., & Marcussi, S. (2021). Lipases and proteases inhibition by Averrhoa carambola L. fruit extracts. Phytomedicine Plus, 1(4), 100119. https://doi.org/10.1016/j.phyplu.2021.100119

Patil, A. G., Koli, S. P., & Patil, D. A. (2013). Pharmcognostical Standardization and HPTLC Fingerprint of Averrhoa bilimbi (L.) Fruits. Journal of Pharmacy Research, 6(1), 145–150. https://doi.org/10.1016/j.jopr.2012.11.030

Pertiwi, D., Hafiz, I., Jannah, W., Winata, H. S., Sari, M., & Suroyo, R. B. (2020a). Antibacterial activities of belimbing wuluh (Averrhoa bilimbi l.) ethyl acetate extract on gel formulated against propionibacterium acnes and staphylococcus aureus. International Journal of Applied Pharmaceutics, 12(6), 224–228. https://doi.org/10.22159/ijap.2020v12i6.39406

Plaper, A., Golob, M., Hafner, I., Oblak, M., Šolmajer, T., & Jerala, R. (2003). Characterization of quercetin binding site on DNA gyrase. Biochemical and Biophysical Research Communications, 306(2), 530–536. https://doi.org/10.1016/S0006-291X(03)01006-4

Rahmiati, A., Darmawati, S., & Mukaromah, A. H. (2017). Inhibition of Ethanol Extract of Carambola Fruit (Averrhoa bilimbi L.) Against the Growth of Staphylococcus aureus and Staphylococcus epidermidis In Vitro. Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian Dan Pengabdian Masyarakat, 30 September 2017, 669–674.

Sá, R. D., Vasconcelos, A. L., Santos, A. V., Padilha, R. J. R., Alves, L. C., Soares, L. A. L., & Randau, K. P. (2019). Anatomy, histochemistry and oxalic acid content of the leaflets of Averrhoa bilimbi and Averrhoa carambola. Revista Brasileira de Farmacognosia, 29(1), 11–16. https://doi.org/10.1016/j.bjp.2018.09.005

Sari, L., Jusuf, N. K., & Putra, I. B. (2020). Bacterial Identification of Acne Vulgaris. Bali Medical Journal, 9(3), 753–756. https://doi.org/10.15562/bmj.v9i3.1737

Scania, A. E., & Chasani, A. R. (2021). The anti-bacterial effect of phenolic compounds from three species of marine macroalgae. Biodiversitas, 22(6), 3412–3417. https://doi.org/10.13057/biodiv/d220649

Seebaluck-Sandoram, R., Lall, N., Fibrich, B., Blom van Staden, A., Saleem, H., & Mahomoodally, M. F. (2019). Antimicrobial, antioxidant and cytotoxic evaluation of two underutilised food plants: Averrhoa bilimbi L. (Oxalidaceae) and Phyllanthus acidus L. Skeels (Phyllanthaceae). Biocatalysis and Agricultural Biotechnology, 18(1), 1–19. https://doi.org/10.1016/j.bcab.2019.01.036

Setyawan, H. Y., Sukardi, S., & Nareswari, B. F. (2021). The phytochemical potential of Averrhoa bilimbi - A review. IOP Conference Series: Earth and Environmental Science, 733(1). https://doi.org/10.1088/1755-1315/733/1/012091

Shamsudin, N. F., Ahmed, Q. U., Mahmood, S., Shah, S. A. A., Khatib, A., Mukhtar, S., Alsharif, M. A., Parveen, H., & Zakaria, Z. A. (2022). Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules, 27(4), 1–43. https://doi.org/10.3390/molecules27041149

Stapleton, P. D., Shah, S., Hamilton-Miller, J. M. T., Hara, Y., Nagaoka, Y., Kumagai, A., Uesato, S., & Taylor, P. W. (2004). Anti-Staphylococcus aureus activity and oxacillin resistance modulating capacity of 3-O-acyl-catechins. International Journal of Antimicrobial Agents, 24(4), 374–380. https://doi.org/10.1016/j.ijantimicag.2004.03.024

Yan, Y., Li, X., Zhang, C., Lv, L., Gao, B., & Li, M. (2021). Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids : A Review. Antibiotics, 10(318), 1–30. https://doi.org/https://doi.org/10.3390/antibiotics 10030318 Academic

Zarwinda, I., Fauziah, F., Jumirna, J., & Adriani, A. (2021). The Formulation of Peel-Off Mask From The Ethanol Extract of Bilimbi Leaves (Averrhoa blimbi L.) As Anti-Acne Treatment. Lantanida Journal, 9(1). https://doi.org/10.22373/lj.v9i1.10206




DOI: https://doi.org/10.14421/biomedich.2024.131.173-182

Refbacks

  • There are currently no refbacks.




Copyright (c) 2024 Reza Anindita, Dede Dwi Nathalia, Melania Perwitasari, Intan Kurnia Putri, Maya Uzia Beandrade, Nofria Rizki Amalia Harahap



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC