Effectiveness Analysis of Antidiabetic Property from Dragon Fruit Peel Methanol Extract in Alloxan-Induced Diabetic Rats

Sonia Apriani Sumartin, I Nyoman Ehrich Lister, Edy Fachrial

Abstract


Diabetes is a health burden in various countries, one of these countries, is also Indonesia. Dragon fruit peel is an alternative diabetes therapy that has been widely studied. Therefore, this study aimed to investigate an antidiabetic effect of dragon fruit peel methanol extract on alloxan-induced diabetic male Wistar rats. This experimental study used twenty-five male Wistar rats induced by alloxan injection. After 48 hours, all rats were grouped into five different groups, including control (0.5% SCMC), standard (Metformin), Dragon Fruit Peel Methanol Extract-1 (500 mg/kg BW), 2 (750 mg/kg BW), and 3 (1,000 mg/kg BW). These treatments were given for two weeks. After that, all rats were dissected to obtain the pancreas. The results showed that dragon fruit peel methanol extract significantly decreased blood glucose levels after the 7th and 14th days (P value <0.05). In addition, pancreatic histology showed a decrease in the extract dose followed by a smaller size of the pancreatic Langerhans islet. The lowest dose of the extract showed a similar size of pancreatic Langerhans islet to the control group with an atrophic pancreatic Langerhans islet. Therefore, it can be concluded that dragon fruit peel extract can significantly decrease blood glucose levels and improve the structure of pancreatic Langerhans islet at higher doses.

Keywords


Anti Diabete; Dragon Fruit Peel; Langerhans Islet; Pancreas

Full Text:

PDF

References


Chiuman, L. (2019). Protective Effect of Curcuma zedoria Against Copper in Rats’ White Blood Cell. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 59(1), 142–148.

Chiuman, L., Ginting, C. N., Yulizal, O., Suhartomi, & Chiuman, V. (2021). Improvement of Liver Function from Lemon Pepper Fruit Ethanol Extract in Streptozotocin-Induced Wistar Rats. 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce). https://doi.org/10.1109/InHeNce52833.2021.9537284

Depari, S. A. F., Jihan, D., Rambe, A., Meilando, R., Lisya, C., Mutia, M. S., Eliza, Y., & Lubis, P. (2021). Uji Efektivitas Ekstrak Etanol Kulit Jeruk Sunkist (Citrus sinensis ( L .) Osbeck) Terhadap Kadar Gula Darah Tikus Wistar (Rattus norvegicus) Dengan Hiperkolestrolemia. Biospecies, 14(1), 1–9.

Dewangan, H., Tiwari, R. K., Sharma, V., Shukla, S. S., Satapathy, T., & Pandey, R. (2017). Past and future of in-vitro and in-vivo animal models for diabetes: A review. Indian Journal of Pharmaceutical Education and Research, 51(4S), S522–S530. https://doi.org/10.5530/ijper.51.4s.79

Elfahmi, Woerdenbag, H. J., & Kayser, O. (2014). Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. Journal of Herbal Medicine, 4(2), 51–73. https://doi.org/10.1016/j.hermed.2014.01.002

Ighodaro, O. M., Adeosun, A. M., & Akinloye, O. A. (2017a). Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina (Lithuania), 53(2017), 365–374. https://doi.org/10.1016/j.medici.2018.02.001

Ighodaro, O. M., Adeosun, A. M., & Akinloye, O. A. (2017b). Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina (Lithuania), 53(6), 365–374. https://doi.org/10.1016/j.medici.2018.02.001

Internation Diabetes Federation. (2019). IDF Diabetes Atlas Ninth (Ninth). International Diabetes Federation.

Kanon, M. Q., Fatimawati, & Bodhi, W. (2012). Uji Efektivitas Ekstrak Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) terhadap Penurunan Kadar Gula Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus L.) yang Diinduksi Sukrosa. Pharmacon, 1(2), 52–58.

Karthikeyan, M., Balasubramanian, T., & Kumar, P. (2016). In-vivo Animal Models and In-vitro Techniques for Screening Antidiabetic Activity. Journal of Developing Drugs, 5(153), 1–6. https://doi.org/10.4172/2329-6631.1000153

Mahargyani, W. (2019). Uji Aktivitas Antidiabetes Ekstrak n-Heksan Kulit Buah Naga Merah (Hylocereus polyrhyzus). EduChemia (Jurnal Kimia dan Pendidikan), 4(1), 13–23. https://doi.org/10.30870/educhemia.v4i1.3958

Mutia, M. S. (2019). Histology Study of Liver Changes Paracetamol-Induced Wistar Rats Treated with Sunkist (Citrus sinensis L . Osbeck) Extract. American Scientific Research Journal for Engineering, Technology, and Science, 59(1), 1–7.

Mutia, M. S., & Chiuman, L. (2019). Efek Hepatoprotektif Ekstrak Etanol Citrus sinensis L . Osbeck pada Tikus yang Diinduksi Parasetamol [Hepatoprotective Effect of Citrus sinensis ( L .) Osbeck Ethanol Extract in Paracetamol-Induced Rats]. Majalah Kedokteran Bandung, 51(4), 189–193.

Nair, A., & Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7(2), 27. https://doi.org/10.4103/0976-0105.177703

Njagi E N Mwaniki, M. J. M., & Njagi J Murugi, N. M. P. (2015). In Vivo Anti-diabetic Effects of Aqueous Leaf Extracts of Rhoicissus tridentata in Alloxan Induced Diabetic Mice. Journal of Developing Drugs, 04(03), 1–5. https://doi.org/10.4172/2329-6631.1000131

Panjaitan, R. G. P., & Novitasari. (2021). Anti-diabetic activity of the Red Dragon Fruit Peel (Hylocereus polyrhizus) in ethanol extract against diabetic rats. Pharmacognosy Journal, 13(5), 1079–1085. https://doi.org/10.5530/pj.2021.13.140

Purnamasari, D. (2014). Diagnosis dan Klasifikasi Diabetes Melitus. In S. Setiati, I. Alwi, A. W. Sudoyo, M. S. K, B. Setiyohadi, & A. F. Syam (Ed.), Buku Ajar Ilmu Penyakit Dalam Jilid I Edisi VI. Interna Publishing.

Putri, M. D., Wiboworini, B., & Dirgahayu, P. (2021). Red dragon fruit juice in reducing ros levels and insulin resistance In rats with type 2 diabetes mellitus model. Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition), 10(1), 6–14. https://doi.org/10.14710/jgi.10.1.6-14

Solikhah, T. I., Rani, C. A., Septiani, M., Putra, Y. A., Rachmah, Q., Solikhah, G. P., Agustono, B., Yunita, M. N., & Purnama, M. T. (2022). Antidiabetic of Hylocereus polyrhizus peel ethanolic extract on alloxan induced diabetic mice. Iraqi Journal of Veterinary Sciences, 36(3), 797–802. https://doi.org/10.33899/ijvs.2022.132178.2061

Suhartomi, Gulo, K. N., Saragih, A. D., Martinus, A. R., & Ikhtiari, R. (2020). Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract. Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, 371–378. https://doi.org/10.5220/0009515503710378

Vijayaraj, R., Kumaran, N. S., & Swarnakala. (2019). In vivo and In vitro Models for Biological Screening of Anti-Diabetic Drugs. International Journal of Pharmacy and Sciences, 9(2), 294–286. https://doi.org/10.21276/ijpbs.2019.9.2.39

Widowati, W., Rani, A. P., Amir Hamzah, R., Arumwardana, S., Afifah, E., Kusuma, H. S. W., Rihibiha, D. D., Nufus, H., & Amalia, A. (2017). Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Natural Product Sciences, 23(3), 192–200. https://doi.org/10.20307/nps.2017.23.3.192

Zubaidah, E., Afgani, C. A., Kalsum, U., Srianta, I., & Blanc, P. J. (2019). Comparison of in vivo antidiabetes activity of snake fruit Kombucha, black tea Kombucha and metformin. Biocatalysis and Agricultural Biotechnology, 17(November 2018), 465–469. https://doi.org/10.1016/j.bcab.2018.12.026




DOI: https://doi.org/10.14421/biomedich.2024.131.57-62

Refbacks

  • There are currently no refbacks.




Copyright (c) 2024 Sonia Apriani Sumartin, I Nyoman Ehrich Lister, Edy Fachrial



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC