Antibacterial Potency and Physicochemical Profiles of Eucalyptus pellita Leaf Waste Essential Oil from PT Surya Hutani Jaya, East Kalimantan

Wartomo Wartomo, Farida Aryani, Muhammad Fikri Hernandi, Erna Rositah, Sri Ngapiyatun, Nur Maulida Sari

Abstract


Eucalyptus is one of the fast growing species plants, currently used in the pulp and paper industry. The leaves of Eucalyptus are known as forest harvesting waste. This research aim to examine the antibacterial potency and the physicochemical profiles of Eucalyptus pellita leaves waste essential oils grown in PT Surya Hutani Jaya Site 32, East Kalimantan named EP B077. The essential oils distilled using water and steam distillation methods. Analysis of physicochemical profiles from this oil included yield, colour, refractive index, specific gravity, and chemical compositions by GC-MS. Antibacterial activity assayed by agar diffusion method against Staphylococcus epidermidis, Streptoccoccus sobrinus and Streptoccoccus mutans with slight modification. The results showed that EP B077 had the yellow colour, yield oils was 0.44%, 1.471 of refractive index, and 0.8706 of specific gravity. Chemical components of EP B077 oils were dominated by α-Pinene (40.36%), 1-β-Pinene (31.75%), Cyclohexene, 1-methyl-4-(1-methylethenyl)- (9.64%), Trans(β)-Caryophyllene (4.29%) and Eucalyptol (1,8-Cineole) (3.88%). This EP B077 oils was active to inhibit bacteria against S. epidermidis and S. sobrinus range from 9-15 mm, also against S.mutans ranging from 9-14 mm. Based on the results, this Eucalyptus pellita leaves named EP B077 had potential to develop as a new source of essential oil and antibacterial agents.

Keywords


Eucalyptus pellita; essential oils; antibacterial; α-Pinene; 1-β-Pinene

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References


Aldoghaim, F. S., Flematti, G. R., & Hammer, K. A. (2018). Antimicrobial activity of several cineole-rich western australian eucalyptus essential oils. Microorganisms, 6(4). https://doi.org/10.3390/microorganisms6040122

Aleksic Sabo, V., & Knezevic, P. (2019). Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. In Industrial Crops and Products (Vol. 132, pp. 413–429). Elsevier B.V. https://doi.org/10.1016/j.indcrop.2019.02.051

Álvarez-Martínez, F. J., Barrajón-Catalán, E., Herranz-López, M., & Micol, V. (2021). Antibacterial plant compounds, extracts and essential oils: An updated review on their effects and putative mechanisms of action. In Phytomedicine (Vol. 90). Elsevier GmbH. https://doi.org/10.1016/j.phymed.2021.153626

Aoki, Y., Van Trung, N., & Suzuki, S. (2019). Impact of piper betle leaf extract on grape downy mildew: Effects of combining 4-allylpyrocatechol with eugenol, α-pinene or β-pinene. Plant Protection Science, 55(1), 23–30. https://doi.org/10.17221/53/2018-PPS

Bachir, R. G., & Benali, M. (2012). Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pacific Journal of Tropical Biomedicine, 2(9), 739–742. https://doi.org/10.1016/S2221-1691(12)60220-2

Boukhatem, M. N., Boumaiza, A., Nada, H. G., Rajabi, M., & Mousa, S. A. (2020). Eucalyptus globulus essential oil as a natural food preservative: Antioxidant, antibacterial and antifungal properties in vitro and in a real food matrix (orangina fruit juice). Applied Sciences (Switzerland), 10(16). https://doi.org/10.3390/app10165581

Dawood, M. A. O., El Basuini, M. F., Zaineldin, A. I., Yilmaz, S., Hasan, M. T., Ahmadifar, E., El Asely, A. M., Abdel-Latif, H. M. R., Alagawany, M., Abu-Elala, N. M., Van Doan, H., & Sewilam, H. (2021). Antiparasitic and antibacterial functionality of essential oils: An alternative approach for sustainable aquaculture. In Pathogens (Vol. 10, Issue 2, pp. 1–38). MDPI AG. https://doi.org/10.3390/pathogens10020185

El Atki, Y., Aouam, I., El Kamari, F., Taroq, A., Nayme, K., Timinouni, M., Lyoussi, B., & Abdellaoui, A. (2019). Antibacterial activity of cinnamon essential oils and their synergistic potential with antibiotics. Journal of Advanced Pharmaceutical Technology and Research, 10(2), 63–67. https://doi.org/10.4103/japtr.JAPTR_366_18

Guimarães, A. C., Meireles, L. M., Lemos, M. F., Guimarães, M. C. C., Endringer, D. C., Fronza, M., & Scherer, R. (2019). Antibacterial activity of terpenes and terpenoids present in essential oils. Molecules, 24(13). https://doi.org/10.3390/molecules24132471

Hii, S. Y., Ha, K. S., Ngui, M. L., Ak Penguang, S., Duju, A., Teng, X. Y., & Meder, R. (2017). Assessment of plantation-grown Eucalyptus pellita in Borneo, Malaysia for solid wood utilisation. Australian Forestry, 80(1), 26–33. https://doi.org/10.1080/00049158.2016.1272526

Hou, J., Zhang, Y., Zhu, Y., Zhou, B., Ren, C., Liang, S., & Guo, Y. (2019). Α-Pinene induces apoptotic cell death via caspase activation in human ovarian cancer cells. Medical Science Monitor, 25, 6631–6638. https://doi.org/10.12659/MSM.916419

Insuan, W., & Chahomchuen, T. (2020). Chemical composition and antimicrobial activity of essential oil extracted from eucalyptus citriodora leaf. Microbiology and Biotechnology Letters, 48(2), 148–157. https://doi.org/10.4014/mbl.1912.12016

Julianus Sohilait, H. (2016). GC/GC-MS Analysis, Isolation and Identification of Bark Essential Oil Components from <i>Cinnamomum culilawan</i>, Blume. American Journal of Applied Chemistry, 4(4), 157. https://doi.org/10.11648/j.ajac.20160404.16

Kartiko, A. B., Putri, A. S., Rosamah, E., & Kuspradini, H. (2021). Evaluation of Antibacterial Activity and Physico-Chemical Profiles of Eucalyptus pellita Essential Oil from East Kalimantan.

Khammassi, M., Polito, F., Amri, I., Khedhri, S., Hamrouni, L., Nazzaro, F., Fratianni, F., & De Feo, V. (2022). Chemical Composition and Phytotoxic, Antibacterial and Antibiofilm Activity of the Essential Oils of Eucalyptus occidentalis, E. striaticalyx and E. stricklandii. Molecules, 27(18). https://doi.org/10.3390/molecules27185820

Kuspradini, H., Putri, A. S., Egra, S., & Yanti. (2019). Short communication: In vitro antibacterial activity of essential oils from twelve aromatic plants from East Kalimantan, Indonesia. Biodiversitas, 20(7), 2039–2042. https://doi.org/10.13057/biodiv/d200733

Kuspradini, H., Sinta Putr, A., & Mitsunaga, T. (2018). Chemical Composition, Antibacterial and Antioxidant Activities of Essential Oils of Dryobalanops lanceolata Burck. Leaf. Research Journal of Medicinal Plants, 12(1), 19–25. https://doi.org/10.3923/rjmp.2018.19.25

Limam, H., Ben Jemaa, M., Tammar, S., Ksibi, N., Khammassi, S., Jallouli, S., Del Re, G., & Msaada, K. (2020). Variation in chemical profile of leaves essential oils from thirteen Tunisian Eucalyptus species and evaluation of their antioxidant and antibacterial properties. Industrial Crops and Products, 158. https://doi.org/10.1016/j.indcrop.2020.112964

McEwan, A., Marchi, E., Spinelli, R., & Brink, M. (2020). Past, present and future of industrial plantation forestry and implication on future timber harvesting technology. In Journal of Forestry Research (Vol. 31, Issue 2, pp. 339–351). Northeast Forestry University. https://doi.org/10.1007/s11676-019-01019-3

Miguel, M., Gago, C., Antunes, M., Lagoas, S., Faleiro, M., Megías, C., Cortés-Giraldo, I., Vioque, J., & Figueiredo, A. (2018). Antibacterial, Antioxidant, and Antiproliferative Activities of Corymbia citriodora and the Essential Oils of Eight Eucalyptus Species. Medicines, 5(3), 61. https://doi.org/10.3390/medicines5030061

Mutlu-Ingok, A., Devecioglu, D., Dikmetas, D. N., Karbancioglu-Guler, F., & Capanoglu, E. (2020). Antibacterial, antifungal, antimycotoxigenic, and antioxidant activities of essential oils: an updated review. In Molecules (Vol. 25, Issue 20). MDPI AG. https://doi.org/10.3390/molecules25204711

Rafiei, V., Ruffino, A., Persson Hodén, K., Tornkvist, A., Mozuraitis, R., Dubey, M., & Tzelepis, G. (2022). A Verticillium longisporum pleiotropic drug transporter determines tolerance to the plant host β-pinene monoterpene. Molecular Plant Pathology, 23(2), 291–303. https://doi.org/10.1111/mpp.13162




DOI: https://doi.org/10.14421/biomedich.2023.122.693-697

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Copyright (c) 2024 Wartomo, Farida Aryani, Muhammad Fikri Hernandi, Erna Rositah, Sri Ngapiyatun, Nur Maulida Sari



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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