Antidiarrhoeal Activities of Lime (Citrus aurantiifolia) Extract in Experimentally-Induced Diarrhoea Model

Joshua Charles Isirima, Precious Ojo Uahomo


This study investigated the effects of Lime on Diarrhoea in Wistar rats. A total of 60 Wistar rats were procured and randomly divided into 3 groups of 20 animals each for each of the three t-test models. The twenty healthy Wistar rats for each diarrhoea model were fasted for 6 hours prior to the experiment but allowed free access to water. The twenty animals were randomly divided into 5 groups of 4 animals each for each experiment. Established antidiarrhea models were followed. The test groups received various doses (97.65mg/kg, 195.3mg/kg, and 390.6mg/kg) of Citrus aurantiifolia juice extract; whereas positive controls received Loperamide (2.5mg/kg) and negative controls received distilled water (1ml/kg). The administration was done once daily for 15 days, and the faeces of each animal was collected on the 5th, 10th and 15th day. The result of this study showed that medium and high dose Citrus aurantiifolia has an anti-diarrhoeal effect on castor oil-induced diarrhoea over repeated administration for a minimum of 15 days as it prolonged the onset of diarrhoea, decreased the frequency of defecation and gastrointestinal transit time in Wistar rats. This study shows that Citrus aurantiifolia demonstrates significant anti-diarrhoeal activity and can be used as an anti-diarrhoea agent.


Diarrhoea; Citrus aurantiifolia; Castor Oil; Lime juice extract; Gastrointestinal Tract; Enteropooling; Diarrhoea model

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Agegnehu MD, Zeleke LB, Goshu YA,Ortibo YL, Mehretie AY. (2019). Diarrhea prevention practice and associated factors among caregivers of under-five children in Enemay district, Northwest Ethiopia. Journal of Environmental Public Health; 5490716.

Akanji MA, Yakubu MT (2000). ?-tocopherol protects against metabisulphite-induced tissue damage in rats. Nigerian Journal of Biochemistry & Molecular Biology; 15(2):179183.

Amita P, Shalini T (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry; 2 (5): 115-119

Atta AH, Mouneir SM (2004) Antidiarrhoeal activity of some Egyptian medicinal plant extracts Journal of Ethnopharmacology 92 (2-3), 303-309, 2004

Audu R., Umilabug S. A., Renner J. K., Awodiji J. A. (2000). Diarrhoea management. Journal of Nigerian Infection Control Association; 3:1517.

Bajad S., Bedi K. L., Singla A. K., Johri R. K. Antidiarrhoeal activity of piperine in mice. Planta Medica. 2001;67(3):284287.

Baker DE (2007). Loperamide: a pharmacological review. Rev Gastroenterol Disord.; 7 Suppl 3: S11S18.

Bern C, Martines J, De Zoysa I, Glass RI (1992). The magnitude of the global problem of diarrhoeal disease: A ten-year update. Bulletin of World Health Organization,70:705-714.

Bina LJ, Tista P, Anjana S and Kayo DY. (2010). Study of antimicrobial activity of lime juice against Vibrio cholerae. Scientific World, 8(8), 44-46

Brijesh S., Tetali P., Birdi T. J. (2011). Study of effect of anti-diarrhoeal medicinal plants on enteropathogenic Escherichia coli induced interleukin-8 secretion by intestinal epithelial cells. Alternative Medicine Studies; 1(1, article e16) doi:

Chitme HR, Chandra R, (2004). Kaushik S. Studies on anti-diarrhoeal activity of Calotropis gigantean R. Br. in experimental animals. Journal of Pharmacy & Pharmaceutical Sciences; 7(1):7075.

D'Aquino, Miguel T, Sergio, A (1994). Lemon juice as a natural biocide for disinfecting drinking water Data

Das K, Tiwari RKS and Shrivastava DK (2010). Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research, 4(2):104-111.

Ekawati ER, Darmanto W (2019). Lemon (Citrus limon) Juice Has Antibacterial Potential against Diarrhoea-Causing Pathogen. IOP Conf. Series: Earth and Environmental Science, 217 012023.

Ekor M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacology, 4:177.

Giday, M., Asfaw, Z., & Woldu, Z. (2010). Ethnomedicinal study of plants used bySheko ethnic group of Ethiopia. Journal ofethnopharmacology, 132(1), 7585.

Grant J. P. (1993). The State of the World Children. UNICEF; Diarrhoeal disease: a strategy for the 90s; pp. 2223.

Hardman J. G., Limbird L. E., Goodman F., Gilman M. (2001). The Pharmacological Basis of Therapeutics. 10th. New York, NY, USA: McGraw-Hill.

Hutton G., Haller L., Bartram J. (2007). Global cost-benefit analysis of water supply and sanitation interventions. Journal of Water and Health.; 5(4):481501.

Hussain Z, Amresh G, Singh S, Rao CV.2009. Antidiarrhoeal and antiulcer activity of Amaranthus spinosus in experimental animals. Pharmaceutical Biology; 47(10):932-939

Jouret-Mourin, K. and Geboes, A. (2002) Infectious Colitis. Acta Endoscopica, 32, 167-183.

Keusch GT, Joan W, Arthur K. (2006). Stigma and global health: developing a research agenda. The Lancet, 367 (9509), 525-527

Kola-Mustapha AT, Ghazali YO, Ayotunde HT, Atunwa SA and Usman SO (2019). Evaluation of the antidiarrheal activity of the leaf extract of Parquetinanigrescens and formulation into oral suspensions: Journal of Experimental Pharmacology, 11; 65-72

Lin J, Puckree T, Mvelase TP (2002). Anti-diarrhoeal evaluation of some medicinal plants used by Zulu traditional healers. Journal of Ethnopharmacology 79 (1), 53-56

Lutterodt G. D. (1992). Inhibition of Microlax-induced experimental diarrhoea with narcotic-like extracts of Psidium guajava leaf in rats. Journal of ethnopharmacology, 37(2), 151157.

Olawuyi JF, Egbewale BE, Anifalaje LA, Okochi EA. (2004). Care seeking practices on diarrhoea in a rural community in Nigeria. African Journal of clinical and experimental microbiology 5 (1), 119-125

Prastiwi SS and Ferdiansyah F (2017). Kandungan Dan Aktivitas Farmakologi Jeruk Nipis (Citrus aurantifolia Swing), Farmaka, 15(2), 18. Tersedia pada:

Susan EA, Mays A. (2005). Pharmacology. In: The Merck veterinary manual. 9th ed. USA: Merck and Co. Inc; p. 1638.

Suleiman MM, Dzenda T, Sani CA (2008). Antidiarrhoeal activity of the methanol stem-bark extract of Annona senegalensis Pers. (Annonaceae) Journal of Ethnopharmacology, 116(1), 125-130

Snyder, J.D. & Merson, M. H. (1982). The magnitude of the global problem of acute diarrhoeal disease: a review of the active surveillance data. Bulletin of the World Health Organization, 60: 605-613

Sow, S. O., Muhsen, K., Nasrin, D., Blackwelder, W. C., Wu, Y., Farag, T. H., Panchalingam, S., Sur, D., Zaidi, A. K., Faruque, A. S., Saha, D., Adegbola, R., Alonso, P. L., Breiman, R. F., Bassat, Q., Tamboura, B., Sanogo, D., Onwuchekwa, U., Manna, B., Ramamurthy, T., Levine, M. M. (2016). The Burden of Cryptosporidium Diarrhoeal Disease among Children < 24 Months of Age in Moderate/High Mortality Regions of Sub-Saharan Africa and South Asia, Utilizing Data from the Global Enteric Multicenter Study (GEMS). PLoS neglected tropical diseases, 10(5), e0004729.

Sastry N., Burgard S. (2005). The prevalence of diarrhoeal disease among Brazilian children: trends and differentials from 1986 to 1996. Social Science & Medicine; 60(5):923935.

Schuster BG (2001). A new integrated program for natural product development and the value of an ethnomedical approach. Journal of Alternative and Complementary Medicine, 7(1):61-72.

Teke GN, Kuiate JR, Ngouateu OB, Gatsing D (2007). Antidiarrhoeal and antimicrobial activities of Emilia coccinea (Sims) G. Don extracts. Journal of ethnopharmacology 112 (2), 278-283

Than A, Kulkarni HJ, Hmone W, Tha SJ.1989. Anti-diarrhoeal efficacy of some Burmese indigenous drug formulations in experimental diarrhoeal test models. International Journal of Crude Drug Research, 27:195200

Uahomo PO and Isirima JC (2022). Antidiarrheal Properties of Aqueous leaf extract of Cyathula prostrata on Castor oil-induced Diarrhoea in Wistar Rats. International Journal of Pharmaceutical Research and Applications, 7(4),1679-1692

UNICEF/WHO (2009). Final Report Diarrhoea. Geneva, Switzerland: United Nations Childrens Fund/World Health Organization. Why children are still dying and what can be done.

UNICEF (2019). DiarrhoeaUNICEF DATA. [(accessed on 29 October 2021)]. Available online:

World Health Organization (2006). Country Health System Fact Sheet. Available via: Accessed 20th December, 2022.



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Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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