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Chemical Composition and Evaluation of Anti-tyrosinase and Anti-Oxidative Effects of Topical Cream Formulation from Acacia sieberiana, Vitellaria paradoxa and Beeswax
Abstract
Skin diseases can get natural therapies from medicinal plant-based products. In this study, a topical cream was formulated from ethanol extract of Acacia sieberiana, beeswax and Vitellaria paradoxa (shea) butter. GC-MS characterization with co-injection of the topical cream revealed Stearic acid (31.43%), Palmitic acid (23.15%), Oleic acid (21.44%) and Linoleic acid (16.20%) as the major components. Seven phenolic conpounds were identified and quantified by HPLC- DAD and Ferulic acid (12.810.26 mg/g) was the most abundant. The cream showed good antioxidant properties evaluated through ?-Carotene-linoleic acid assay, DPPH radical scavenging, ABTS+ assay, CUPRAC assay, and metal chelating assay. The cream had higher activity in the DPPH assay (IC50 = 32.100.84 g/mL), ABTS+ assay (IC50 = 22.490.62 g/mL) and CUPRAC assay (IC50 = 49.270.79 g/mL) than ?-Tocopherol. The antioxidant effects are an indication that the cream can reduce oxidative stress on the skin including aging, carcinogenesis and inflammation. At 100 g/mL, the topical cream showed tyrosinase inhibition of 48.230.87% regarded as relatively good compared to the standard tyrosinase inhibitor kojic acid, which showed 79.500.32% inhibition at the same concentration. The cosmetic cream was able to inhibit the melanin production rate-limiting enzyme, tyrosinase, indicating that it can control hyperpigmentation and skin spots.
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DOI: https://doi.org/10.14421/biomedich.2023.121.251-258
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Copyright (c) 2023 Alfred Ngenge Tamfu, Alain Yaya Koudoro, Selcuk Kucukaydin, Theophile Olaye, Pascal Dossa Cokou Agbangnan, Dominique Codjo Koko Sohounhloue, Felicien Avlessi
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