High Performance Liquid Chromatography (HPLC) for Detection of Glucosamine and Chondroitin Sulfate Compounds

Rakhmiyati Rakhmiyati, Tetri Widiyani, Agung Budiharjo

Abstract


Glucosamine and chondroitin sulfate are compounds found in shark cartilage (Carcharhinus sorrah). The two compounds have many health benefits, that is wound healing and helping the process of angiogenesis. This study aims to determine the content of glucosamine and chondroitin sulfate compounds in shark cartilage (SC) extract. The method used was High Performance Liquid Chromatography (HPLC) with potassium phosphate buffer solution at pH 3. The results of this research were SC extract contained glucosamine and chondroitin sulfate compounds with a retention time of 1.914 minutes.

Keywords


HPLC; shark; glucosamine; chondroitin sulfate

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References


Agiba, A.M. (2017). Nutraceutical Formulations Containing Glucosamine and Chondroitin Sulphate In The Treatment Of Osteoarthritis: Emphasis On Clinical Efficacy And Formulation Challenges. International Journal of Current Pharmaceutical R esearch 9 (2): 1-7.

American College of Rheumatology Subcommittee on Osteoarthritis Guidelines. (2000). ACR recommendations for the medical management of osteoarthritis of the hip and knee: 2000 update. Arthritis Rheum 43:1905-1915.

Barclay, T.S., Tsourounis, C., and McCart, G.M. (1998). Glucosamine. The Annals of Pharmacotherapy 32: 574-579.

Bargahi A and A. Rabbani-Chadegani A. (2008). Angiogenic inhibitor protein fractions derived from shark cartilage. Journal Biochemical Society 28 (1): 15–21.

Dahmer, S. & Schiller, R.M. (2008). Glucosamine. American Family Physician 78 (4): 471-476.

Dean MN and AP Summers. (2006). Mineralized Cartilage in the skeleton of chondrichthyan fishes. Zoology 109 (2): 164-168.

De Mattei, M., Pellati, A., Pasello, M., De Terlizzi, F., Massari, L., Gemmati, D., & Caruso, A. (2002). High doses of glucosamine-HCl have detrimental effects on bovine articular cartilage explants cultured in vitro. Osteoarthritis and Cartilage 10 (10): 816–825.

González, R.P., Leyva, A. & Moraes, M.O. (2001). Shark Cartilage as Source of Antiangiogenic Compounds: From Basic to Clinical Research. Biol. Pharm. Bull. 24 (10): 1097-1101.

Henrotin, Y., Mathy, M., Sanchez, C., & Lambert, C. (2010). Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations. Therapeutic Advances in Musculoskeletal Disease 2 (6): 335-348.

Huskisson, E.C. (2008). Glucosamine and Chondroitin for Osteoarthritis. The Journal of International Medical Research 36(6): 1-19.

Hutagalung, E. (2005). Current status of glucosamine and chondroitin therapy in osteoarthritis. Med J Indones. 14 (1): 55-58.

Jerosch, J. (2011). Effects of Glucosamine and Chondroitin Sulfate on Cartilage Metabolism in OA: Outlook on Other Nutrient Partners Especially Omega-3 Fatty Acids. International Journal of Rheumatology. p:1-17.

Kerri l. Pettrey, ML Cupp, and TS Tracy. (2003). Dietary Supplements: Toxicology and Clinical Pharmacology. Edited by: MJ Cupp and TS Tracy © Humana Press Inc., Totowa, New Jersey.

Lippiello, L. (2003). Glucosamine and Chondroitin sulfate: Biological Response Modifiers of Chondrocytes Under Simulated Conditions of Joint Stress. Osteoarthritis and Cartilage 11 (5): 335-342.

Lubis, A.M.T., Siagian, C., Wonggokusuma, E., Marsetio, A.F., Setyohadi, B. (2017). Comparison of Glucosamine-Chondroitin Sulfate with and without Methylsulfonylmethane in Grade I-II Knee Osteoarthritis: A Double Blind Randomized Controlled Trial. Acta Med Indones-Indones J Intern Med 49 (2): 105-111.

Martel-Pelletier J, A Farran, E Montell, J Verges, JP Pelletier. (2015). Discrepancies in Composition and Biological Effects of Different Formulations of Chondroitin Sulfate. Molecules 20 (3): 4277-4289.

Marzuki AS, Kasim dan N Arafah. (2014). Pengaruh Penambahan Natrium Hidroksida Terhadap Waktu Retensi Kadar Kondroitin Sulfat dari Tulang Rawan Ikan Pari (Taeniura lymna). Jurnal Phinisi 9 (3): 239-245.

Matsuno, H., Nakamura, H., Katayama, K., Hayashi, S., Kano, S., Yudoh, K., & Kiso, Y. (2009). Effects of an Oral Administration of GlucosamineChondroitin-Quercetin Glucoside on the Synovial Fluid Properties in Patients with Osteoarthritis and Rheumatoid Arthritis. Bioscience, Biotechnology, and Biochemistry 73 (2): 288–292.

Miller KL and DO Clegg. (2011). Glucosamine and Chondroitin Sulfate. Rheumatic Diseases Clinics of North America 37 (1): 103–118.

Nakano, Ikawa and Ozimek. (2000). An economical method to extract chondroitin sulphatepeptide from bovine nasal cartilage. Canadian Agricultural Engineering 42 (4): 205-208.

Pavelká K, Gatterová J, Olejarová M, Machacek, S., Giacovelli, G., Rovati, L.C. (2002). Glucosamine sulfate use and delay of progression of knee osteoarthritis: a 3-year, randomized, placebo-controlled, double-blind study. Arch Intern Med 162: 2113 – 2123.

Pelletier, J.M., Farran, A., Montell, E., Vergés, J., and Pelletier, J.P. (2015). Discrepancies in Composition and Biological Effects of Different Formulations of Chondroitin Sulfate. Molecules 20: 4277-4289.

Shmagel, A., Demmer, R., Knights, D., Butler, M., Langsetmo, L., Lane, N.E., and Ensrud, K. (2019). The Effects of Glucosamine and Chondroitin Sulfate on Gut Microbial Composition: A Systematic Review of Evidence from Animal and Human Studies. Nutrients 11 (2): 1-14.

Siagian C. (2014). Efek Pemberian Kombinasi Glukosamin-Kondroitin Sulfat, Kombinasi Glukosamin-Kondroitin Sulfat-Methylsulfonylmethane, dan Plasebo Terhadap Kesembuhan Pasien Osteoarthritis Sendi Lutut Derajat Kellgren Lawrence I dan II (Uji Klinis Acak Tersamar Ganda). Tesis. Jakarta: Universitas Indonesia.

Šimánek, V., Křen, V., Ulrichová, J., Gallo, J. (2005). The Efficacy Of Glucosamine And Chondroitin Sulfate In The Treatment Of Osteoarthritis: Are These Saccharides Drugs Or Nutraceuticals?. Biomed. Papers 149(1): 51–56.

Sulityowati, W., Titiek Indhira, A., Arbai, A., and Yatmasari, E. (2015). Glucosamine and Chondroitin Sulphate Content of Shark Cartilage (Prionace glauca) and its Potential as Anti-Aging Supplements. International Journal of ChemTech Research 8 (10): 163-168.

Toffoletto, O., Tavares, A., Casarini, D.E., Redublo, B.M., Ribeiro, A.B. (2005). Pharmacokinetic Profile of Glucosamine and Chondroitin Sulfate Association in Healthy Male Individuals. Acta Ortop Bras 13(5): 235-237.

Václavíková, E., and Kvasnička, F. Isotachophoretic Determination of Glucosamine and Chondroitin Sulphate in Dietary Supplements. Czech J. Food Sci 31 (1): 55-65.

Vasiliadis, H.S., Tsikopoulos, K. (2017). Glucosamine and chondroitin for the treatment of Osteoarthritis. World Journal Orthopedics 8 (1): 1-11.

Widyaningsih TD, WD Rukmi, E Sofia, SD Wijayanti, N Wijayanti, R Ersalia, N Rochmawati, D Nangin. (2016). Extraction of Glycosaminoglycans Containing Glucosamine and Chondroitin Sulfate from Chicken Claw Cartilage. Research Journal of Life Science 3 (3): 181-189.

Xie, J., HY Ye and XF Luo. (2014). An effcient preparation of chondroitin sulfate and collagen peptides from shark cartilage. International Food Research Journal 21(3): 1171-1175.




DOI: https://doi.org/10.14421/biomedich.2023.121.5-8

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Copyright (c) 2022 Rakhmiyati, Tetri Widiyani, Agung Budiharjo



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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